Stim2 antibody (Center)

Catalog No. ABIN500831

The Rabbit Polyclonal anti-Stim2 antibody has been validated for WB, IHC (p) and EIA. It is suitable to detect Stim2 in samples from Human, Mouse and Rat.

Quick Overview for Stim2 antibody (Center) (ABIN500831)

Target: Stim2 (Stromal Interaction Molecule 2 (Stim2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Stim2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-Stim2 Antibody

Binding Specificity: Center

Specificity: This antibody detects STIM2 at center. It is predicted to have no cross-reactivity to STIM1.

Cross-Reactivity (Details): Species reactivity (tested):Human, mouse, rat

Purification: Peptide affinity chromatography

Immunogen: STIM2 antibody was raised against a 17 amino acid peptide from near the center of human STIM2.

Isotype: IgG

Application Details

Application Notes: ELISA. Western blot: 0.5 - 1 μg/mL. Immunohistochemistry on paraffin sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Target Details for Stim2

Target: Stim2 (Stromal Interaction Molecule 2 (Stim2))

Alternative Name: STIM2

Background: In T lymphocytes, the sole pathway for Ca++ entry following antigen-receptor binding is through store-operated Ca++-release-activated Ca++ (CRAC) channels. These channels are made up of the pore-forming subunit ORAI1 and the stromal interaction molecule 1 (STIM1), a protein that functions as a Ca++ sensor and activates the CRAC channels, migrating to the plasma membrane from endoplasmic reticulum (ER)-like sites which act as the Ca++ store. A related molecule, STIM2, acts to inhibit the STIM1-mediated store-operated Ca++ entry, and can form complexes with STIM1, suggesting they may play a coordinated role in controlling Ca++ entry. At least three isoforms of STIM2 are known to exist. This STIM2 antibody is predicted to have no cross-reactivity to STIM1.Synonyms: KIAA1482, Stromal interaction molecule 2

Gene ID: 57620

NCBI Accession: NP_065911

Pathways: TCR Signaling, BCR Signaling