TMED10 antibody (Center)

Catalog No. ABIN500992

Product Details anti-TMED10 Antibody

Target: TMED10 (Transmembrane Emp24-Like Trafficking Protein 10 (TMED10))

Binding Specificity: Center

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TMED10 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Specificity: This antibody detects TMED10 / TMP21 at Center.

Cross-Reactivity (Details): Species reactivity (tested):Human, mouse, rat

Purification: Peptide affinity chromatography

Immunogen: TMP21 antibody was raised against a 18 amino acid peptide from near the center of human TMP21.

Isotype: IgG

Application Details

Application Notes: ELISA. Western blot: 1 - 2 μg/mL. Immunohistochemistry on paraffin sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Target Details for TMED10

Target: TMED10 (Transmembrane Emp24-Like Trafficking Protein 10 (TMED10))

Alternative Name: TMED10 / TMP21 (TMED10 Products)

Background: TMP21 is a ubiquitously expressed protein that is involved in vesicular targeting and protein transport. More recent experiments have shown that it is also a component in the presenilin complex and modulates the γ-secretase but not theεsecretase cleavage activity of the amyloid precursor protein. The presenilin complex is composed of the proteins APH1, nicastrin, and PEN2 in addition to presenilin-1. Together, these proteins cleave the amyloid precursor protein at what is known as the γ and ε-sites and can lead to the accumulation of the Aβ cleavage product that is associated with Alzheimer's disease. Co-immunoprecipitation experiments using antibodies against these proteins also yielded TMP21 indicating that TMP21 may play a role in the regulation of this complex. Suppression of TMP21 expression by siRNA in transfected cells caused increased γ-secretase activity but not ε-secretase activity, and increased Aβ production, demonstrating that TMP21 can modulate γsecretase activity.Synonyms: 21 kDa transmembrane-trafficking protein, S31I125, Transmembrane emp24 domain-containing protein 10, Transmembrane protein Tmp21

Gene ID: 10972