TRAF3 antibody (N-Term)

Catalog No. ABIN501026

The Rabbit Polyclonal anti-TRAF3 antibody has been validated for WB, IF and EIA. It is suitable to detect TRAF3 in samples from Human, Mouse and Rat.

Quick Overview for TRAF3 antibody (N-Term) (ABIN501026)

Target: TRAF3 (TNF Receptor-Associated Factor 3 (TRAF3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TRAF3 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Product Details anti-TRAF3 Antibody

Binding Specificity: N-Term

Specificity: This antibody detects TRAF3 / CRAF1 at N-term.

Cross-Reactivity (Details): Species reactivity (tested):Human, mouse, rat

Purification: Peptide affinity chromatography

Immunogen: TRAF3 antibody was raised against a 15 amino acid peptide from near the amino terminus of human.

Isotype: IgG

Application Details

Application Notes: ELISA. Western blot: 1 - 2 μg/mL. Immunoflourescence.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Target Details for TRAF3

Target: TRAF3 (TNF Receptor-Associated Factor 3 (TRAF3))

Alternative Name: TRAF3 / CRAF1

Background: Tumor necrosis factor (TNF) receptor associated factors (TRAFs) are the major signal transducers for the TNF receptor superfamily and the interleukin-1 receptor/Toll-like receptor (IL-1/TLR) superfamily (1). TRAF3 was first identified by its interaction with CD40 and the Epstein-Barr virus transforming protein LMP1 (2,3). Several TRAF3 mRNA splice variants exist and some of these can activate the transcription factor NF-kappaB (4). Besides CD40, TRAF3 also interacts with the TRFR superfamily member lymphotoxin-beta receptor (LTbetaR) in association with TRAF2 and the apoptosis inhibitors cIAP1 and Smac. It has been suggested that TRAF3 induces mitochondria-mediated apoptosis upon binding of the TNF family cytokine LIGHT by LTbetaR.Synonyms: CAP-1, CAP1, CD40 receptor-associated factor 1, CD40-binding protein, CD40BP, LAP1, LMP1-associated protein, TNF receptor-associated factor 3

Gene ID: 7187

NCBI Accession: NP_663777

Pathways: NF-kappaB Signaling, Apoptosis, TLR Signaling, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades