XAF1 antibody (C-Term)

Catalog No. ABIN501126

This anti-XAF1 antibody is a Rabbit Polyclonal antibody detecting XAF1 in WB, IHC (p) and EIA. Suitable for Human and Mouse.

Quick Overview for XAF1 antibody (C-Term) (ABIN501126)

Target: XAF1 (XiAP Associated Factor 1 (XAF1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This XAF1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-XAF1 Antibody

Binding Specificity: C-Term

Specificity: This antibody detects XAF-1.

Cross-Reactivity (Details): Species reactivity (tested):Human, mouse

Purification: Affinity chromatography

Immunogen: XAF-1 antibody was raised against a synthetic peptide corresponding to amino acids at the C-terminus of human XAF-1.

Isotype: IgG

Application Details

Application Notes: ELISA. Immunohistochemistry on paraffin sections. Western blot.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Target Details for XAF1

Target: XAF1 (XiAP Associated Factor 1 (XAF1))

Alternative Name: XAF1

Background: XAF-1 binds to XIAP, an inhibitor of caspases-3, -7, and -9, and triggers its relocation from the cytosol to the nucleus (1,2). Overexpression of XAF-1 results in the neutralization of XIAP\'s ability to inhibit cell death (1). XAF-1 is normally expressed in all adult and fetal tissues but was found to be present in very low levels in a variety of cancer cell lines (3). In contrast, XIAP levels have been shown to be high in a majority of cell lines. Low XAF-1 and high basal expression of XIAP may therefore play a critical role in maintaining survival of cancer cell lines (2,3). Both IFN-alpha2 and IFN-beta can induce XAF-1 mRNA in all cells examined but induction of XAF-1 protein (as observed by immunoblot analysis) was seen only in cell lines sensitive to the apoptotic effects of IFNs (4).Synonyms: BIRC4-binding protein, BIRC4BP, XIAP-associated factor 1, XIAPAF1

Gene ID: 54739

NCBI Accession: NP_059993