Topo IIalpha (pSer1106) antibody (AbBy Fluor® 750)

Catalog No. ABIN5011804

The Rabbit Polyclonal anti- antibody has been validated for WB, IF (cc) and IF (p). It is suitable to detect in samples from Human and Zebrafish (Danio rerio).

Quick Overview for Topo IIalpha (pSer1106) antibody (AbBy Fluor® 750) (ABIN5011804)

Target: Topo IIalpha

Reactivity: Human, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: AbBy Fluor® 750

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details

Binding Specificity: pSer1106

Cross-Reactivity: Human, Zebrafish (Danio rerio)

Predicted Reactivity: Dog,Sheep,Pig

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human TOPO II Alpha around the phosphorylation site of Ser1106

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: Topo IIalpha

Alternative Name: TOPO II Alpha

Background:

Synonyms: DNA topoisomerasealpha, DNA topoisomerase 2-alpha, DNA topoisomerase II, alpha isozyme, TOP2, TP2A, TOP2A, DNA topoisomerase II 170 kD, DNA topoisomerase II alpha isozyme, DNA Topoisomerase2, TOP 2A, TopII alpha, Topo II alpha, Topoisomerase DNA II alpha 170 kDa, Topoisomerase II alpha 170 kDa, TOP2A_HUMAN.

Background: This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, alpha, is localized to chromsome 17 and the beta gene is localized to chromosome 3. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia.

Gene ID: 7153