Sonic Hedgehog antibody (AA 237-399)

Catalog No. ABIN5013678

The Rabbit Polyclonal anti-Sonic Hedgehog antibody has been validated for WB, IHC, IP and ICC. It is suitable to detect Sonic Hedgehog in samples from Rat.

Quick Overview for Sonic Hedgehog antibody (AA 237-399) (ABIN5013678)

Target: Sonic Hedgehog (SHH)

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Sonic Hedgehog antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-Sonic Hedgehog Antibody

Binding Specificity: AA 237-399

Purpose: Polyclonal Antibody to Hedgehog Homolog, Sonic (SHH)

Specificity: The antibody is a rabbit polyclonal antibody raised against SHH. It has been selected for its ability to recognize SHH in immunohistochemical staining and western blotting.

Cross-Reactivity: Human, Mouse

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: SHH (Ser237-Arg399)

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.2-2 μg/mL,1:250-2500 Immunohistochemistry: 5-20 μg/mL,1:25-100 Immunocytochemistry: 5-20 μg/mL,1:25-100 Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: PBS, pH 7.4, containing 0.01 % SKL, 1 mM DTT, 5 % Trehalose and Proclin300.

Preservative: Dithiothreitol (DTT), ProClin

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Target Details for Sonic Hedgehog

Target: Sonic Hedgehog (SHH)

Alternative Name: Hedgehog Homolog, Sonic

Background: HHG1, HLP3, HPE3, SMMCI, SHHN, SHH-N

Pathways: Hedgehog Signaling, Dopaminergic Neurogenesis, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development