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Sonic Hedgehog antibody (AA 237-399)

SHH Reactivity: Rat WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN5013678
  • Target See all Sonic Hedgehog (SHH) Antibodies
    Sonic Hedgehog (SHH)
    Binding Specificity
    • 14
    • 11
    • 9
    • 8
    • 6
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 237-399
    Reactivity
    • 78
    • 51
    • 38
    • 15
    • 9
    • 6
    • 5
    • 4
    • 4
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Rat
    Host
    • 79
    • 21
    • 6
    • 1
    • 1
    Rabbit
    Clonality
    • 72
    • 36
    Polyclonal
    Conjugate
    • 66
    • 8
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This Sonic Hedgehog antibody is un-conjugated
    Application
    • 80
    • 38
    • 29
    • 19
    • 18
    • 12
    • 12
    • 12
    • 10
    • 8
    • 4
    • 3
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)
    Purpose
    Polyclonal Antibody to Hedgehog Homolog, Sonic (SHH)
    Specificity
    The antibody is a rabbit polyclonal antibody raised against SHH. It has been selected for its ability to recognize SHH in immunohistochemical staining and western blotting.
    Cross-Reactivity
    Human, Mouse
    Purification
    Antigen-specific affinity chromatography followed by Protein A affinity chromatography
    Immunogen
    SHH (Ser237-Arg399)
    Isotype
    IgG
    Top Product
    Discover our top product SHH Primary Antibody
  • Application Notes
    Western blotting: 0.2-2 μg/mL,1:250-2500 Immunohistochemistry: 5-20 μg/mL,1:25-100 Immunocytochemistry: 5-20 μg/mL,1:25-100 Optimal working dilutions must be determined by end user.
    Comment

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    500 μg/mL
    Buffer
    PBS, pH 7.4, containing 0.01 % SKL, 1 mM DTT, 5 % Trehalose and Proclin300.
    Preservative
    Dithiothreitol (DTT), ProClin
    Storage
    4 °C,-20 °C
    Storage Comment
    Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
  • Target
    Sonic Hedgehog (SHH)
    Alternative Name
    Hedgehog Homolog, Sonic (SHH Products)
    Background
    HHG1, HLP3, HPE3, SMMCI, SHHN, SHH-N
    Pathways
    Hedgehog Signaling, Dopaminergic Neurogenesis, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development
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