HCK antibody (AA 2-524)

Catalog No. ABIN5013688

This anti-HCK antibody is a Rabbit Polyclonal antibody detecting HCK in WB, IHC, IP and ICC. Suitable for Mouse.

Quick Overview for HCK antibody (AA 2-524) (ABIN5013688)

Target: HCK (Hemopoietic Cell Kinase (HCK))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HCK antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-HCK Antibody

Binding Specificity: AA 2-524

Purpose: Polyclonal Antibody to Hemopoietic Cell Kinase (HCK)

Specificity: The antibody is a rabbit polyclonal antibody raised against HCK. It has been selected for its ability to recognize HCK in immunohistochemical staining and western blotting.

Cross-Reactivity: Human, Rat

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: HCK (Gly2-Pro524)

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.2-2 μg/mL,1:250-2500 Immunohistochemistry: 5-20 μg/mL,1:25-100 Immunocytochemistry: 5-20 μg/mL,1:25-100 Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Target Details for HCK

Target: HCK (Hemopoietic Cell Kinase (HCK))

Alternative Name: Hemopoietic Cell Kinase

Background: JTK9, Hematopoietic cell kinase, p59-HCK/p60-HCK

Pathways: Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization, CXCR4-mediated Signaling Events, Thromboxane A2 Receptor Signaling