Ubiquitin antibody (Atto 594)

Catalog No. ABIN5024446

The Mouse Monoclonal anti-Ubiquitin antibody has been validated for WB, IHC, ELISA, ICC and IF. It is suitable to detect Ubiquitin in samples from Human.

Quick Overview for Ubiquitin antibody (Atto 594) (ABIN5024446)

Target: Ubiquitin (Ub)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Ubiquitin antibody is conjugated to Atto 594

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF)

Clone: FK1

Product Details anti-Ubiquitin Antibody (Atto 594)

Specificity: Detects polyubiquitinated proteins and ubiquitin chains. Does not detect monoubiquitinated proteins nor free ubiquitin.

Purification: Gel filtration

Immunogen: Ubiquitin conjugated lysozyme

Isotype: IgM

Application Details

Application Notes:

• WB (1:10000)
• optimal dilutions for assays should be determined by the user.

Comment:

A 1:5000 dilution of SMC-213 was sufficient for detection of ubiquitin conjugates in 2.5 μg of HeLa cell lysate by electrochemiluminescence analysis using goat anti-mouse IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: 10 mM phosphate buffer, 0.15M NaCl pH 7.4, 0.1 % sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Conjugated antibodies should be stored at 4°C

Target Details for Ubiquitin

Target: Ubiquitin (Ub)

Alternative Name: Ubiquitin

Background: Ubiquitin is a small protein found ubiquitously in all tissue types and acts as a post translational modification. It can bind to its substrate either as a single ubiquitin molecule or in a chain. It is involved in many regulatory processes, which include proteasomal degradation, signal transduction, DNA repair, endocytosis and autophagy.

Gene ID: 7314

UniProt: P0CG47

Pathways: Mitotic G1-G1/S Phases, Ubiquitin Proteasome Pathway