BRD4 antibody (AA 140-500)

Catalog No. ABIN5027061

This Rabbit Polyclonal antibody specifically detects BRD4 in WB. It exhibits reactivity toward Human.

Quick Overview for BRD4 antibody (AA 140-500) (ABIN5027061)

Target: BRD4 (Bromodomain Containing 4 (BRD4))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BRD4 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-BRD4 Antibody

Binding Specificity: AA 140-500

Purification: Protein A Chromatography

Immunogen: A partial length recombinant BRD4 protein (amino acids 140-500) was used as the immunogen for this antibody.

Isotype: IgG

Application Details

Application Notes: WB: 2-4 μg/mL This antibody detects ~80 kDa band. The size corresponds to Isoform 2 (Uniprot ID-O60885-2) of BRD4, HUNK1, the right size is ~152 kDa O60885 O60885

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: PBS containing 0.05 % BSA, PH 7.4

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.

Target Details for BRD4

Target: BRD4 (Bromodomain Containing 4 (BRD4))

Alternative Name: BRD4, HUNK1

Background: BRD4 is a member of the BET (bromo domain and extra terminal domain) family, along with BRD2, BRD3 and BRDT. BRD4 is required to form the transcriptionally active P-TEFb complex by displacing negative regulators such as HEXIM1 and 7SKsnRNA complex from P-TEFb, thereby transforming it into an active form that can then phosphorylate the C-terminal domain (CTD) of RNA polymerase II. A chromosomal aberration involving BRD4 is found in a rare, aggressive and lethal carcinoma arising in midline organs of young people. It expressed ubiquitously and has three isoforms. Isoform B acts as a chromatin insulator in the DNA damage response pathway.

Molecular Weight: 88 kDa

Gene ID: 23476

UniProt: O60885

Pathways: Chromatin Binding, SARS-CoV-2 Protein Interactome