PD-L1 antibody (AA 13-224)

Catalog No. ABIN5027497

The Mouse Monoclonal anti-PD-L1 antibody has been validated for WB, FACS and IHC. It is suitable to detect PD-L1 in samples from Human. There is 1 publication available.

Quick Overview for PD-L1 antibody (AA 13-224) (ABIN5027497)

Target: PD-L1 (CD274 (PD-L1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PD-L1 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (IHC)

Clone: ABM5F25

Product Details anti-PD-L1 Antibody

Binding Specificity: AA 13-224

Purification: Protein G Chromatography

Immunogen: A partial length recombinant protein of PD-L1 (amino acid 13-224) was used as the immunogen for this antibody.

Isotype: IgG2b kappa

Application Details

Application Notes: FACS: 0.5-1 μg/10^6 cells, WB: 2-4 μg/mL, IHC: 5-10 μg/mL

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: PBS containing 0.05 % BSA, PH 7.4

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.

References for anti-PD-L1 antibody (ABIN5027497)

Faruk, Ibrahim, Aminu, Adamu, Abdullahi, Suleiman, Rafindadi, Mohammed, Iliyasu, Idoko, Saidu, Randawa, Musa, Ntekim, Shah, Abubakar, Adoke, Manko, Awasum: "Prognostic significance of BIRC7/Livin, Bcl-2, p53, Annexin V, PD-L1, DARC, MSH2 and PMS2 in colorectal cancer treated with FOLFOX chemotherapy with or without aspirin." in: PLoS ONE, Vol. 16, Issue 1, pp. e0245581, (2021) (PubMed).

Target Details for PD-L1

Target: PD-L1 (CD274 (PD-L1))

Alternative Name: PDL-1

Background: PD-L1 (CD274/B7-H1) is a critical membrane-bound costimulatory molecule belongs to the B7 superfamily that inhibits immune responses through its receptor, PD-1 and PD-L1 play a key role in the pathogenesis of inflammatory diseases (programmed death 1). It is widely expressed in the mononuclear phagocyte system (MPS), may co-stimulate T cells and regulates inflammatory responses. PD-L1 exerts inflammation regulatory functions via a negative co-stimulatory effect on T cell functions to inhibit cytokine secretion, facilitate apoptosis of activated T cells and induce T cell anergy. Aberrant expression and dysregulation of CD274 have been reported during bacterial infection, inflammation and in numerous autoimmune diseases.

Molecular Weight: 44 kDa

Gene ID: 29126

UniProt: Q9NZQ7

Pathways: Cancer Immune Checkpoints