14-3-3 sigma/SFN antibody

Catalog No. ABIN5027687

The Rabbit Polyclonal anti-14-3-3 sigma/SFN antibody (ABIN5027687) specifically detects 14-3-3 sigma/SFN in WB and IHC. The antibody is reactive with Human samples.

Quick Overview for 14-3-3 sigma/SFN antibody (ABIN5027687)

Target: 14-3-3 sigma/SFN (SFN) (Stratifin (SFN))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This 14-3-3 sigma/SFN antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-14-3-3 sigma/SFN Antibody

Purification: Protein A Chromatography

Immunogen: Full length recombinant 14-3-3 sigma protein was used as the immunogen for this antibody.

Isotype: IgG

Application Details

Application Notes: WB: 1-3 μg/mL, IHC: 10 μg/mL

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: PBS containing 0.05 % BSA, PH 7.4

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.

Target Details for 14-3-3 sigma/SFN

Target: 14-3-3 sigma/SFN (SFN) (Stratifin (SFN))

Alternative Name: 14-3-3 sigma

Background: 14-3-3 sigma is a member of a highly conserved family of 14-3-3 proteins that are present in all eukaryotic organisms. There are 7 known human 14-3-3 isotypes and they play important roles in many biological activities by binding to and altering the subcellular localization and/or stability of key molecules in various signaling cascades. 14-3-3 sigma was originally characterized as a human mammary epithelium marker 1 and later rediscovered as an important molecule for cell cycle checkpoint regulation. Recently it has been reported that 14-3-3 sigma may serve as a prognosis marker predicting survival of pancreatic cancer patient treatment. 14-3-3 is the only 14-3-3 isoform induced by the tumor suppressor protein p53, in response to -irradiation and other DNA-damaging agents. 14-3-3 is a p53-regulated inhibitor of G2/M progression and acts as a tumor suppressor gene that is inactivated by methylation of its 5' CpG islands in epithelial tumor cells. Two isoforms of the human proteins are produced by alternative splicing.

Molecular Weight: 32 kDa

Gene ID: 2810

UniProt: P31947

Pathways: p53 Signaling, Myometrial Relaxation and Contraction