ULK1 antibody (AA 567-577)

Catalog No. ABIN5066137

The Rabbit Polyclonal anti-ULK1 antibody has been validated for WB, IF and ICC. It is suitable to detect ULK1 in samples from Human.

Quick Overview for ULK1 antibody (AA 567-577) (ABIN5066137)

Target: ULK1 (Unc-51 Like Kinase 1 (ULK1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ULK1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-ULK1 Antibody

Binding Specificity: AA 567-577

Specificity: Ubiquitously expressed. Detected in the following adult tissues: skeletal muscle, heart, pancreas, brain, placenta, liver, kidney, and lung.,Detects ~100 kDa.

Cross-Reactivity: Human, Mouse, Rat

Purification: Peptide Affinity Purified

Immunogen: Synthetic peptide from the mid-protein of Human ULK1 (aa. 567-577)

Isotype: IgG

Application Details

Application Notes:

• WB (1:1000)
• ICC/IF (1:100)
• optimal dilutions for assays should be determined by the user.

Comment:

A 1:1000 dilution of ABIN5066137 was sufficient for detection of ULK1 in 15 μg of Human HeLa Cell Lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: -20°C

Target Details for ULK1

Target: ULK1 (Unc-51 Like Kinase 1 (ULK1))

Alternative Name: ULK1

Background: UNC-51 like kinase 1 (ULK1) is widely expresed and contains an amino-terminal kinase domain followed by a central proline-serine rich domain and a highly conserved carboxy-terminal domain. It has been linked to axon growth and is essential for autophagy. Structurally, ULK1 is similar to ATG1, and it appears that both Atg1/ULK1 can bind to several ATG proteins regulating phosphorylation states and protein trafficking.

Gene ID: 8408

NCBI Accession: NP_003556

UniProt: O75385

Pathways: Regulation of Cell Size, Autophagy