ATG9B antibody (AA 110-121) (Biotin)

Catalog No. ABIN5066220

This Rabbit Polyclonal antibody specifically detects ATG9B in WB, ICC and IF. It exhibits reactivity toward Human.

Quick Overview for ATG9B antibody (AA 110-121) (Biotin) (ABIN5066220)

Target: ATG9B (Autophagy Related 9B (ATG9B))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATG9B antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)

Product Details anti-ATG9B Antibody (Biotin)

Binding Specificity: AA 110-121

Specificity: Highly expressed in placenta (trophoblast cells) and pituitary gland. Not expressed in vascular endothelial.,Detects ~100 kDa.

Cross-Reactivity: Human, Mouse

Purification: Peptide Affinity Purified

Immunogen: Synthetic peptide from the N-terminal of Human ATG9B (aa. 110-121)

Isotype: IgG

Application Details

Application Notes:

• WB (1:1000)
• ICC/IF (1:100)
• optimal dilutions for assays should be determined by the user.

Comment:

A 1:1000 dilution of ABIN5066220 was sufficient for detection of ATG9B in 15 μg of Human HeLa Cell Lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Conjugated antibodies should be stored at 4°C

Target Details for ATG9B

Target: ATG9B (Autophagy Related 9B (ATG9B))

Alternative Name: ATG9B

Background: ATG9B is involved in autophagy and cytoplasm to vacuole transport (Cvt) vesicle formation. Plays a key role in the organization of the preautophagosomal structure/phagophore assembly site (PAS), the nucleating site for formation of the sequestering vesicle. Highly expressed in the placenta and the pituitary gland.

Gene ID: 285973

NCBI Accession: NP_001303985

UniProt: Q674R7