SCNN1A antibody (AA 46-68)

Catalog No. ABIN5066857

The Mouse Monoclonal anti-SCNN1A antibody is suitable to detect SCNN1A in samples from Rat. It has been validated for WB and IHC.

Quick Overview for SCNN1A antibody (AA 46-68) (ABIN5066857)

Target: SCNN1A (Sodium Channel, Nonvoltage-Gated 1 alpha (SCNN1A))

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This SCNN1A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: 2G4

Product Details anti-SCNN1A Antibody

Binding Specificity: AA 46-68

Specificity: Detects ~85 kDa.

Cross-Reactivity: Mouse, Rat

Purification: Protein G Purified

Immunogen: Synthetic peptide from the N-terminal of Rat ENaC alpha (aa. 46-68)

Isotype: IgG2b

Application Details

Application Notes:

• WB (1:1000)
• IHC (1:150)
• optimal dilutions for assays should be determined by the user.

Comment:

A 1:1000 dilution of ABIN5066857 was sufficient for detection of ENaC alpha in 15 μg of Mouse whole kidney lysate by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: -20°C

Target Details for SCNN1A

Target: SCNN1A (Sodium Channel, Nonvoltage-Gated 1 alpha (SCNN1A))

Alternative Name: ENaC alpha

Background: The Epithelial Sodium Channel (ENaC) is a membrane ion channel permeable to Na+ ions. It is located in the apical plasma membrane of epithelia in the kidneys, lung, colon, and other tissues where it plays a role in trans epithelial Na+-ion transport (1). Specifically Na+ transport via ENaC occurs across many epithelial surfaces, and plays a key role in regulating salt and water absorption (2). ENaCs are composed of three structurally related subunits that form a tetrameric channel, alpha, beta, and gamma. The expression of its alpha and beta subunits is enhanced as keratinocytes differentiate (3, 4). The beta and gamma-ENaC subunits are essential for edema fluid to exert its maximal effect on net fluid absorption by distal lung epithelia(5). And it has been concluded that the subunits are differentially expressed in the retina of mice with ocular hypertension, therefore the up-regulation of alpha-ENaC proteins could serve as a protection mechanism against elevated intraocular pressure (6).

Gene ID: 25122

NCBI Accession: NP_113736

UniProt: Q6IRJ1