7-Ketocholesterol (7-KC) antibody (Atto 488)

Catalog No. ABIN5067309

This Mouse Monoclonal antibody specifically detects in ELISA, ICC, IF and WB. It exhibits reactivity toward .

Quick Overview for 7-Ketocholesterol (7-KC) antibody (Atto 488) (ABIN5067309)

Target: 7-Ketocholesterol (7-KC)

Host: Mouse

Clonality: Monoclonal

Conjugate: Atto 488

Application: ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)

Clone: 7E1

Product Details

Specificity: Specific for 7-ketocholesterol modified proteins.

Purification: Protein G Purified

Immunogen: Synthetic 7-Ketocholesterol conjugated to Keyhole Limpet Hemocyanin (KLH).

Isotype: IgG2b

Application Details

Application Notes:

• WB (1:1000)
• ICC/IF (1:50)
• ELISA (1:1000)
• optimal dilutions for assays should be determined by the user.

Comment:

A 1:1000 dilution of ABIN5067309 was sufficient for detection of 7-Ketocholesterol in 2 μg of 7-ketocholesterol conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % Sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Conjugated antibodies should be stored at 4°C

Target Details

Target: 7-Ketocholesterol (7-KC)

Alternative Name: 7-Ketocholesterol

Target Type: Hormone

Background: Lipid peroxidation occurs when oxidizing agents attack carbon-carbon double bonds found in unsaturated lipids. In addition to membrane degradation, oxidation end-products have been found to damage cell viability through their mutagenic and toxic properties. These downstream functional consequences facilitate the development of disease and premature aging. 7-ketocholesterol (7-KC) is a biomarker of lipoprotein cholesterol oxidation.