CrkL antibody (AA 204-303)

Catalog No. ABIN514606

This Mouse Monoclonal antibody specifically detects CrkL in WB, ELISA, IF, PLA and RNAi. It exhibits reactivity toward Human and has been mentioned in 2+ publications.

Quick Overview for CrkL antibody (AA 204-303) (ABIN514606)

Target: CrkL (CRKL) (V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian)-Like (CRKL))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CrkL antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Proximity Ligation Assay (PLA), RNA Interference (RNAi)

Clone: 4B5

Product Details anti-CrkL Antibody

Binding Specificity: AA 204-303

Purpose: Mouse monoclonal antibody raised against a partial recombinant CRKL.

Sequence: AHAYAQPQTT TPLPAVSGSP GAAITPLPST QNGPVFAKAI QKRVPCAYDK TALALEVGDI VKVTRMNING QWEGEVNGRK GLFPFTHVKI FDPQNPDENE

Cross-Reactivity: Human

Characteristics: Antibody Reactive Against Recombinant Protein.

Immunogen: CRKL (NP_005198, 204 a.a. ~ 303 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Isotype: IgG2b

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: In 1x PBS, pH 7.4

Handling Advice: Aliquot to avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

References for anti-CrkL antibody (ABIN514606)

Liu, Chen, Chau, Jan, Chen, Hsu, Lin, Juang, Lu, Cheng, Chen, Chang, Ting, Kao, Hsiao, Huang: "Analysis of protein-protein interactions in cross-talk pathways reveals CRKL protein as a novel prognostic marker in hepatocellular carcinoma." in: Molecular & cellular proteomics : MCP, Vol. 12, Issue 5, pp. 1335-49, (2013) (PubMed).

Segovis, Schoon, Dick, Nacusi, Leibson, Billadeau: "PI3K links NKG2D signaling to a CrkL pathway involved in natural killer cell adhesion, polarity, and granule secretion." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 182, Issue 11, pp. 6933-42, (2009) (PubMed).

Target Details for CrkL

Target: CrkL (CRKL) (V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian)-Like (CRKL))

Alternative Name: CRKL

Background: Full Gene Name: v-crk sarcoma virus CT10 oncogene homolog (avian)-like
Synonyms:

Gene ID: 1399

NCBI Accession: NM_005207

Pathways: Interferon-gamma Pathway, Neurotrophin Signaling Pathway, Signaling of Hepatocyte Growth Factor Receptor