EN1 antibody (AA 266-392)

Catalog No. ABIN515285

The Mouse Monoclonal anti-EN1 antibody has been validated for WB, ELISA and IHC (p). It is suitable to detect EN1 in samples from Human. There is 1 publication available.

Quick Overview for EN1 antibody (AA 266-392) (ABIN515285)

Target: EN1 (Engrailed Homeobox 1 (EN1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This EN1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 3H2

Product Details anti-EN1 Antibody

Binding Specificity: AA 266-392

Purpose: Mouse monoclonal antibody raised against a full length recombinant EN1.

Sequence: SQQPLVWPAW VYCTRYSDRP SSGPRTRKLK KKKNEKEDKR PRTAFTAEQL QRLKAEFQAN RYITEQRRQT LAQELSLNES QIKIWFQNKR AKIKKATGIK NGLALHLMAQ GLYNHSTTTV QDKDESE*

Cross-Reactivity: Human

Characteristics: Antibody Reactive Against Recombinant Protein.

Immunogen: EN1 (NP_001417, 266 a.a. ~ 392 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Isotype: IgG2a

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: In 1x PBS, pH 7.4

Handling Advice: Aliquot to avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

References for anti-EN1 antibody (ABIN515285)

Veenvliet, Dos Santos, Kouwenhoven, von Oerthel, Lim, van der Linden, Koerkamp, Holstege, Smidt: "Specification of dopaminergic subsets involves interplay of En1 and Pitx3." in: Development (Cambridge, England), Vol. 140, Issue 16, pp. 3373-84, (2013) (PubMed).

Target Details for EN1

Target: EN1 (Engrailed Homeobox 1 (EN1))

Alternative Name: EN1

Background: Full Gene Name: engrailed homeobox 1
Synonyms:

Gene ID: 2019

NCBI Accession: NM_001426

Pathways: Dopaminergic Neurogenesis