GCLM antibody (AA 1-274)

Catalog No. ABIN516058

This Mouse Monoclonal antibody specifically detects GCLM in WB and ELISA. It exhibits reactivity toward Human and has been mentioned in 4+ publications.

Quick Overview for GCLM antibody (AA 1-274) (ABIN516058)

Target: GCLM (Glutamate-Cysteine Ligase, Modifier Subunit (GCLM))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This GCLM antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Clone: 2B8

Product Details anti-GCLM Antibody

Binding Specificity: AA 1-274

Purpose: Mouse monoclonal antibody raised against a full length recombinant GCLM.

Sequence: MGTDSRAAKA LLARARTLHL QTGNLLNWGR LRKKCPSTHS EELHDCIQKT LNEWSSQINP DLVREFPDVL ECTVSHAVEK INPDEREEMK VSAKLFIVES NSSSSTRSAV DMACSVLGVA QLDSVIIASP PIEDGVNLSL EHLQPYWEEL ENLVQSKKIV AIGTSDLDKT QLEQLYQWAQ VKPNSNQVNL ASCCVMPPDL TAFAKQFDIQ LLTHNDPKEL LSGASFQEAL QESIPDIQAH EWVPLWLLRY SVIVKSRGII KSKGYILQAK RRGS

Cross-Reactivity: Human

Characteristics: Antibody Reactive Against Recombinant Protein.

Immunogen: GCLM (AAH41809, 1 a.a. ~ 274 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Isotype: IgG2a

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: In 1x PBS, pH 7.4

Handling Advice: Aliquot to avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

References for anti-GCLM antibody (ABIN516058)

Sharon, Mor, Golan-lev, Fainsod, Benvenisty: "Molecular and functional characterizations of gastrula organizer cells derived from human embryonic stem cells." in: Stem cells (Dayton, Ohio), Vol. 29, Issue 4, pp. 600-8, (2011) (PubMed).

Abel, Bubel, Simper, Powell, McClellan, Andreeff, MacLeod, DiGiovanni: "Protection against 2-chloroethyl ethyl sulfide (CEES)-induced cytotoxicity in human keratinocytes by an inducer of the glutathione detoxification pathway." in: Toxicology and applied pharmacology, Vol. 255, Issue 2, pp. 176-83, (2011) (PubMed).

Hardwick, Fisher, Canet, Lake, Cherrington: "Diversity in antioxidant response enzymes in progressive stages of human nonalcoholic fatty liver disease." in: Drug metabolism and disposition: the biological fate of chemicals, Vol. 38, Issue 12, pp. 2293-301, (2010) (PubMed).

Balamurugan, Akhov, Selvaraj, Pugazhenthi: "Induction of antioxidant enzymes by curcumin and its analogues in human islets: implications in transplantation." in: Pancreas, Vol. 38, Issue 4, pp. 454-60, (2009) (PubMed).

Target Details for GCLM

Target: GCLM (Glutamate-Cysteine Ligase, Modifier Subunit (GCLM))

Alternative Name: GCLM

Background: Full Gene Name: glutamate-cysteine ligase, modifier subunit
Synonyms: GLCLR

Gene ID: 2730