NT5C2 antibody (AA 1-561)

Catalog No. ABIN524900

The Mouse Monoclonal anti-NT5C2 antibody has been validated for WB, ELISA, IHC (p) and IF. It is suitable to detect NT5C2 in samples from Human. There are 4+ publications available.

Quick Overview for NT5C2 antibody (AA 1-561) (ABIN524900)

Target: NT5C2 (5'-Nucleotidase, Cytosolic II (NT5C2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This NT5C2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Clone: 3C1

Product Details anti-NT5C2 Antibody

Binding Specificity: AA 1-561

Purpose: Mouse monoclonal antibody raised against a full length recombinant NT5C2.

Sequence: MSTSWSDRLQ NAADMPANMD KHALKKYRRE AYHRVFVNRS LAMEKIKCFG FDMDYTLAVY KSPEYESLGF ELTVERLVSI GYPQELLSFA YDSTFPTRGL VFDTLYGNLL KVDAYGNLLV CAHGFNFIRG PETREQYPNK FIQRDDTERF YILNTLFNLP ETYLLACLVD FFTNCPRYTS CETGFKDGDL FMSYRSMFQD VRDAVDWVHY KGSLKEKTVE NLEKYVVKDG KLPLLLSRMK EVGKVFLATN SDYKYTDKIM TYLFDFPHGP KPGSSHRPWQ SYFDLILVDA RKPLFFGEGT VLRQVDTKTG KLKIGTYTGP LQHGIVYSGG SSDTICDLLG AKGKDILYIG DHIFGDILKS KKRQGWRTFL VIPELAQELH VWTDKSSLFE ELQSLDIFLA ELYKHLDSSS NERPDISSIQ RRIKKVTHDM DMCYGMMGSL FRSGSRQTLF ASQVMRYADL YAASFINLLY YPFSYLFRAA HVLMPHESTV EHTHVDINEM ESPLATRNRT SVDFKDTDYK RHQLTRSISE IKPPNLFPLA PQEITHCHDE DDDEEEEEEE E

Cross-Reactivity: Human

Characteristics: Antibody Reactive Against Recombinant Protein.

Immunogen: NT5C2 (AAH01595, 1 a.a. ~ 561 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Isotype: IgG2a

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: In 1x PBS, pH 7.4

Handling Advice: Aliquot to avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

References for anti-NT5C2 antibody (ABIN524900)

Cividini, Tozzi, Galli, Pesi, Camici, Dumontet, Jordheim, Allegrini: "Cytosolic 5'-nucleotidase II interacts with the leucin rich repeat of NLR family member Ipaf." in: PLoS ONE, Vol. 10, Issue 3, pp. e0121525, (2015) (PubMed).

Jordheim, Puy, Cros-Perrial, Peyrottes, Lefebvre, Périgaud, Dumontet: "Determination of the enzymatic activity of cytosolic 5'-nucleotidase cN-II in cancer cells: development of a simple analytical method and related cell line models." in: Analytical and bioanalytical chemistry, Vol. 407, Issue 19, pp. 5747-58, (2015) (PubMed).

Cividini, Cros-Perrial, Pesi, Machon, Allegrini, Camici, Dumontet, Jordheim, Tozzi: "Cell proliferation and drug sensitivity of human glioblastoma cells are altered by the stable modulation of cytosolic 5'-nucleotidase II." in: The international journal of biochemistry & cell biology, Vol. 65, pp. 222-9, (2015) (PubMed).

Kulkarni, Karlsson, Szekeres, Chibalin, Krook, Zierath: "Suppression of 5'-nucleotidase enzymes promotes AMP-activated protein kinase (AMPK) phosphorylation and metabolism in human and mouse skeletal muscle." in: The Journal of biological chemistry, Vol. 286, Issue 40, pp. 34567-74, (2011) (PubMed).

Target Details for NT5C2

Target: NT5C2 (5'-Nucleotidase, Cytosolic II (NT5C2))

Alternative Name: NT5C2

Background: Full Gene Name: 5'-nucleotidase, cytosolic II
Synonyms: GMP,NT5B,PNT5,cN-II

Gene ID: 22978