SMG1 antibody (AA 2922-3031)

Catalog No. ABIN524936

The Mouse Monoclonal anti-SMG1 antibody has been validated for ELISA, WB and IHC (p). It is suitable to detect SMG1 in samples from Human. There are 2+ publications available.

Quick Overview for SMG1 antibody (AA 2922-3031) (ABIN524936)

Target: SMG1 (pi-3-Kinase-Related Kinase SMG-1 (SMG1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This SMG1 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 1A8

Product Details anti-SMG1 Antibody

Binding Specificity: AA 2922-3031

Purpose: Mouse monoclonal antibody raised against a partial recombinant SMG1.

Sequence: PDVMSQNARK LIQKNLATSA DTPPSTVPGT GKSVACSPKK AVRDPKTGKA VQERNSYAVS VWKRVKAKLE GRDVDPNRRM SVAEQVDYVI KEATNLDNLA QLYEGWTAWV

Cross-Reactivity: Human

Characteristics: Antibody Reactive Against Recombinant Protein.

Immunogen: SMG1 (NP_055535, 2922 a.a. ~ 3031 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Isotype: IgG2a

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: In 1x PBS, pH 7.4

Handling Advice: Aliquot to avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

References for anti-SMG1 antibody (ABIN524936)

Arias-Palomo, Yamashita, Fernández, Núñez-Ramírez, Bamba, Izumi, Ohno, Llorca: "The nonsense-mediated mRNA decay SMG-1 kinase is regulated by large-scale conformational changes controlled by SMG-8." in: Genes & development, Vol. 25, Issue 2, pp. 153-64, (2011) (PubMed).

Stalder, Mühlemann: "Processing bodies are not required for mammalian nonsense-mediated mRNA decay." in: RNA (New York, N.Y.), Vol. 15, Issue 7, pp. 1265-73, (2009) (PubMed).

Target Details for SMG1

Target: SMG1 (pi-3-Kinase-Related Kinase SMG-1 (SMG1))

Alternative Name: SMG1

Background: Full Gene Name: SMG1 homolog, phosphatidylinositol 3-kinase-related kinase (C. elegans)
Synonyms: 61E3.4,ATX,KIAA0421,LIP

Gene ID: 23049

NCBI Accession: NM_014006

Pathways: Inositol Metabolic Process