SMT1 antibody

Catalog No. ABIN5326722

This Rabbit Polyclonal antibody specifically detects SMT1 in IL and WB. It exhibits reactivity toward Arabidopsis thaliana and has been mentioned in 2+ publications.

Quick Overview for SMT1 antibody (ABIN5326722)

Target: SMT1 (Cycloartenol-C-24-Methyltransferase (SMT1))

Reactivity: Arabidopsis thaliana

Host: Rabbit

Clonality: Polyclonal

Application: Immunolocalization (IL), Western Blotting (WB)

Product Details anti-SMT1 Antibody

Cross-Reactivity (Details): Not reactive in: Hordeum vulgare, Triticum aestivum, Withania somnifera

Predicted Reactivity: Amborella trichopoda, Brassica napus, Brassica rapa subsp. pekinensis, Capsella rubella, Citrus clementina, Eutrema salsugineum, Glycine max, Glycine soja, Gossypium mexicanum, Medicago truncatula, Populus trichopocarpa, Prunus persica, Ricinus communis, Theobroma cacao, Vitis vinifera,

Characteristics: Expected / apparent Molecular Weight of the Antigene: 38 kDa

Purification: affinity purified

Immunogen: KLH-conjugated synthetic peptide derived from Arabidopsis thaliana SMT1 Q9LM02, At5g13710

Application Details

Application Notes: 1: 500 - 1:1 000 with ECL Advance or + (WB), 1: 50 - 1: 100 (IL)

Comment:

SMT1 antibody characterization in Western Blot and immunofluorescence labeling: Boutté Y et al. (2010). Endocytosis restricts Arabidopsis KNOLLE syntaxin to the cell division plane during late cytokinesis. EMBO J. 29, 5465-5458.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 200 μL of sterile water.

Handling Advice: Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: store lyophilized/reconstituted at -20C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

References for anti-SMT1 antibody (ABIN5326722)

Zhang, Peck: "Simplified enrichment of plasma membrane proteins for proteomic analyses in Arabidopsis thaliana." in: Proteomics, Vol. 11, Issue 9, pp. 1780-8, (2011) (PubMed).

Boutté, Frescatada-Rosa, Men, Chow, Ebine, Gustavsson, Johansson, Ueda, Moore, Jürgens, Grebe: "Endocytosis restricts Arabidopsis KNOLLE syntaxin to the cell division plane during late cytokinesis." in: The EMBO journal, Vol. 29, Issue 3, pp. 546-58, (2010) (PubMed).

Target Details for SMT1

Target: SMT1 (Cycloartenol-C-24-Methyltransferase (SMT1))

Alternative Name: Sterol methyltransferase 1 (SMT1)

Background: AGI Code: At5g13710
SMT1 (EC=2.1.1.41) is an enzyme involved in steroid biosynthesis localized specific to the Endoplasmic Reticulum (ER), Boutte et al., 2009 . It is catalyzing the methyl transfer from S-adenosyl-methionine to the C-24 of cycloartenol to form 24-methylene cycloartenol. Alternative names: Cycloartenol-C-24-methyltransferase, 24-sterol C-methyltransferase 1, Protein STEROL METHYLTRANSFERASE 1, Protein CEPHALOPOD

Molecular Weight: 38 kDa

UniProt: Q9LM02