ACD1 antibody

Catalog No. ABIN5326730

This anti-ACD1 antibody is a Rabbit Polyclonal antibody detecting ACD1 in WB. Suitable for Arabidopsis thaliana.

Quick Overview for ACD1 antibody (ABIN5326730)

Target: ACD1 (Pheophorbide A Oxygenase (ACD1))

Reactivity: Arabidopsis thaliana

Host: Rabbit

Clonality: Polyclonal

Application: Western Blotting (WB)

Product Details anti-ACD1 Antibody

Cross-Reactivity: Arabidopsis thaliana

Cross-Reactivity (Details): dicots including: Brassica napus, Lycopersicum esculentum, Nicotiana tabacum

Predicted Reactivity: dicots including: Brassica napus, Lycopersicum esculentum, Nicotiana tabacum

Characteristics: Expected / apparent Molecular Weight of the Antigene: 61 / 54 kDa
Acd1 (accelerated cell death 1) EC=1.14.12.20 is an enzyme involved in chlorophyll breakdown, pheophorbide a oxygenase. This enzyme seems to induce cell death in Arabidopsis leaves in the dark. Synonymes:PaO, Lls1, lethal leaf-spot 1 homolog, pheide a oxygenase

Purification: serum

Immunogen: recombinant PaO from Arabidopsis thaliana Q9FYC2, At3g44880

Application Details

Application Notes: Recommended Dilution: 1 : 5000 with standard ECL (WB)

Comment:

This antibody works on total cell extracts and can be used as a senescence marker. Predicted size of Acd1 precursor protein is about 61 kD including the transit peptide, but it must be processed to a smaller size. Using fresh extracts is recommended to decrease possible cross-reaction with Rubisco.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Target Details for ACD1

Target: ACD1 (Pheophorbide A Oxygenase (ACD1))

Alternative Name: accelerated cell death 1 (Acd1)

Background: AGI Code: At3g44880
Acd1 (accelerated cell death 1) EC=1.14.12.20 is an enzyme involved in chlorophyll breakdown, pheophorbide a oxygenase. This enzyme seems to induce cell death in Arabidopsis leaves in the dark. Synonymes:PaO, Lls1, lethal leaf-spot 1 homolog, pheide a oxygenase

Molecular Weight: expected: 61 kDa, apparent: 54 kDa

UniProt: Q9FYC2