ACD1 antibody

Catalog No. ABIN5326730

The Rabbit Polyclonal anti-ACD1 antibody (ABIN5326730) specifically detects ACD1 in WB. The antibody is reactive with Arabidopsis thaliana samples.

Quick Overview for ACD1 antibody (ABIN5326730)

Target: ACD1 (Pheophorbide A Oxygenase (ACD1))

Reactivity: Arabidopsis thaliana

Host: Rabbit

Clonality: Polyclonal

Application: Western Blotting (WB)

Product Details anti-ACD1 Antibody

Purpose: Anti-ACD1 | Accelerated cell death 1

Cross-Reactivity: Arabidopsis thaliana

Cross-Reactivity (Details): Arabidopsis thaliana
Not reactive in: Pinus strobus

Predicted Reactivity: Brassica napus,Solanum lycopersicum,Nicotiana tabacum

Purification: Serum

Immunogen: Recombinant PaO from Arabidopsis thaliana Q9FYC2

Application Details

Application Notes: 1 : 5000 (WB)

Comment:

This antibody works on total cell extracts and can be used as a senescence marker. Predicted size of Acd1 precursor protein is about 61 kD including the transit peptide, but it must be processed to a smaller size. Using fresh extracts is recommended to decrease possible cross-reaction with Rubisco.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 50 μL of sterile water

Buffer: Serum

Handling Advice: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Storage: -20 °C

Storage Comment: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Target Details for ACD1

Target: ACD1 (Pheophorbide A Oxygenase (ACD1))

Alternative Name: accelerated cell death 1 (Acd1)

Background:

Background: Acd1 (accelerated cell death 1) EC=1.14.12.20 is an enzyme involved in chlorophyll breakdown, pheophorbide a oxygenase. This enzyme seems to induce cell death in Arabidopsis leaves in the dark. Synonymes:PaO, Lls1, lethal leaf-spot 1 homolog, pheide a oxygenase

Additional information: The protein level is moderately induced during dark-induced senescence

Molecular Weight: 61 | 54 kDa

Gene ID: 823622

UniProt: Q9FYC2