MRPL50 antibody (N-Term)

Catalog No. ABIN5514549

This Rabbit Polyclonal antibody specifically detects MRPL50 in WB. It exhibits reactivity toward Human, Cow, Dog, Guinea Pig, Horse, Mouse and Rat.

Quick Overview for MRPL50 antibody (N-Term) (ABIN5514549)

Target: MRPL50 (Mitochondrial Ribosomal Protein L50 (MRPL50))

Reactivity: Human, Cow, Dog, Guinea Pig, Horse, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MRPL50 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-MRPL50 Antibody

Binding Specificity: N-Term

Sequence: CREFWSRFRK EKEPVVVETV EEKKEPILVC PPLRSRAYTP PEDLQSRLES

Predicted Reactivity: Cow: 85%, Dog: 79%, Guinea Pig: 86%, Horse: 93%, Human: 100%, Mouse: 93%, Rat: 93%

Characteristics: This is a rabbit polyclonal antibody against RM50. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N-terminal region of Human RM50

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for MRPL50

Target: MRPL50 (Mitochondrial Ribosomal Protein L50 (MRPL50))

Alternative Name: RM50

Background: Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75 % protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a putative 39S subunit protein and belongs to the L47P ribosomal protein family. Pseudogenes corresponding to this gene are found on chromosomes 2p, 2q, 5p, and 10q.

Alias Symbols: MRPL50,

Protein Interaction Partner: UBC, MDM2, NPM1, MRPL45, TMEM177, MRPL57, MRPL44, MRPL32, MRPL38, MRPL40, MRPS9, MRPL47, TSR1, RIF1, MRPL37, MRPL15, FLOT1, MRPL19, SUCLG2, SLC25A3, ILF2, ATP6V0A1, ATP6V1B1, CUL3, ICT1,

Protein Size: 158

Gene ID: 54534

NCBI Accession: NP_061924

UniProt: Q8N5N7