REST antibody (N-Term)

Catalog No. ABIN5518090

The Rabbit Polyclonal anti-REST antibody has been validated for WB. It is suitable to detect REST in samples from Human, Rat, Mouse, Cow, Dog, Guinea Pig, Horse, Rabbit and Zebrafish (Danio rerio).

Quick Overview for REST antibody (N-Term) (ABIN5518090)

Target: REST (RE1-Silencing Transcription Factor (REST))

Reactivity: Human, Rat, Mouse, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This REST antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-REST Antibody

Binding Specificity: N-Term

Sequence: SGSSTAEEGD FSKGPIRCDR CGYNTNRYDH YTAHLKHHTR AGDNERVYKC

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 91%

Characteristics: This is a rabbit polyclonal antibody against REST. It was validated on Western Blot.

Purification: Affinity purified

Immunogen: The immunogen is a synthetic peptide directed towards the N-terminal region of Human REST

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for REST

Target: REST (RE1-Silencing Transcription Factor (REST))

Alternative Name: REST

Background: This gene encodes a transcriptional repressor that represses neuronal genes in non-neuronal tissues. It is a member of the Kruppel-type zinc finger transcription factor family. It represses transcription by binding a DNA sequence element called the neuron-restrictive silencer element. The protein is also found in undifferentiated neuronal progenitor cells and it is thought that this repressor may act as a master negative regular of neurogenesis. Alternatively spliced transcript variants have been described.

Alias Symbols: REST, NRSF, XBR,

Protein Interaction Partner: BTRC, UBC, FBXW11, CSNK1E, NEDD8, EHMT2, EIF4A3, FBXW4, FBXW7, FBXW5, FBXW8, FBXW2, SIN3A, TERF2, GRIA2, SUB1, GTF2B, Tbp, SIN3B, HDAC1, RCOR1, SP1, SMARCE1, SMARCC2, SMARCA4, CDYL, HDAC5, HDAC4, HTT, NCOR1, KDM5C, RNF2, HDAC2, STMN2, GRM2, CTDSP1,

Protein Size: 1097

Gene ID: 5978

NCBI Accession: NP_005603

UniProt: Q13127

Pathways: Negative Regulation of Hormone Secretion, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Chromatin Binding, Positive Regulation of Endopeptidase Activity