REST antibody (Internal Region)

Catalog No. ABIN5539667

Product Details anti-REST Antibody

Target: REST (RE1-Silencing Transcription Factor (REST))

Binding Specificity: Internal Region

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This REST antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Purpose: RE1-silencing transcription factor

Sequence: PETPGAEDKG KSSK

Specificity: Reported variants represent identical protein: NP_001180437.1, NP_005603.3

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 140 kDa band observed in nuclear lysates of cell lines HEK293 and HeLa (calculated MW of 122 kDa according to NP_005603.3). This molecular weight is routinely observed by other sources. Recommended concentration: 0.3-1 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:64000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for REST

Target: REST (RE1-Silencing Transcription Factor (REST))

Alternative Name: REST (REST Products)

Synonyms: NRSF antibody, XBR antibody, 2610008J04Rik antibody, AA407358 antibody, xbr antibody, nrsf antibody, rest antibody, resta antibody, REST antibody, RE1 silencing transcription factor antibody, RE1-silencing transcription factor antibody, RE1-silencing transcription factor S homeolog antibody, REST antibody, Rest antibody, rest.S antibody, rest antibody, LOC615792 antibody

Background: REST, RE1-silencing transcription factor, NRSF, XBR, RE1-silencing transcription factor variant E1a/E2/E3/E4c, RE1-silencing transcription factor variant E1a/E2/E3/E5, RE1-silencing transcription factor variant E1a/E2/E3/N3a/E4i, RE1-silencing transcripti

Gene ID: 5978

NCBI Accession: NP_005603

Pathways: Negative Regulation of Hormone Secretion, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Chromatin Binding, Positive Regulation of Endopeptidase Activity