ALOX12 antibody (C-Term)

Catalog No. ABIN5518671

This Rabbit Polyclonal antibody specifically detects ALOX12 in WB. It exhibits reactivity toward Human.

Quick Overview for ALOX12 antibody (C-Term) (ABIN5518671)

Target: ALOX12 (Arachidonate 12-Lipoxygenase (ALOX12))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ALOX12 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-ALOX12 Antibody

Binding Specificity: AA 609-628, C-Term

Purpose: Anti-12 Lipoxygenase/ALOX12 UBB Antibody Picoband®

Sequence: HHKEKYFSGP KPKAVLNQFR

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-12 Lipoxygenase/ALOX12 UBB Antibody (ABIN5518671). Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human ALOX12, different from the related rat sequence by four amino acids, and from the related mouse sequence by five amino acids.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human
1. Funk, C. D., Furci, L., FitzGerald, G. A.Molecular cloning, primary structure, and expression of the human platelet/erythroleukemia cell 12-lipoxygenase.Proc. Nat. Acad. Sci. 87: 5638-5642, 1990. 2. Natarajan, R., Esworthy, R., Bai, W., Gu, J.-L., Wilczynski, S., Nadler, J.Increased 12-lipoxygenase expression in breast cancer tissues and cells. Regulation by epidermal growth factor.J. Clin. Endocr. Metab. 82: 1790-1798, 1997. 3. Yoshimoto, T., Arakawa, T., Hada, T., Yamamoto, S., Takahashi, E.Structure and chromosomal localization of human arachidonate 12-lipoxygenase gene.J. Biol. Chem. 267: 24805-24809, 1992.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Sodium azide, Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for ALOX12

Target: ALOX12 (Arachidonate 12-Lipoxygenase (ALOX12))

Alternative Name: ALOX12

Background:

Synonyms: Polyubiquitin-B,Ubiquitin,UBB,

Tissue Specificity: Expressed in vascular smooth muscle cells.

Background: ALOX12, Arachidonate 12-lipoxygenase, is an enzyme that in humans is encoded by the ALOX12 gene. By fluorescence in situ hybridization, the ALOX12 gene is located in band 17p13.1. The gene consists of 14 exons with 13 introns and spans approximately 15 kb of DNAArachidonate 12-lipoxygenase introduces a molecular oxygen into the C-12 position of arachidonic acid to produce 12 (S)-hydroperoxy-5,8,10,14-eicosatetraenoic acid. The major pathway of arachidonic acid metabolism in human platelets proceeds via a 12-lipoxygenase enzyme. Expression of the LOG12 gene was detected in human erythroleukemia cells, platelets, and human umbilical vein endothelial cells by reverse transcription-PCR analysis.

Sequence Similarities: Belongs to the lipoxygenase family.

Molecular Weight: 75 kDa

Pathways: Positive Regulation of Endopeptidase Activity