COL2A1 antibody (C-Term)

Catalog No. ABIN5518709

This anti-COL2A1 antibody is a Rabbit Polyclonal antibody detecting COL2A1 in WB and IHC. Suitable for Mouse and Rat. This Primary Antibody has been cited in 35+ publications.

Quick Overview for COL2A1 antibody (C-Term) (ABIN5518709)

Target: COL2A1 (Collagen, Type II, alpha 1 (COL2A1))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This COL2A1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-COL2A1 Antibody

Binding Specificity: AA 1222-1241, C-Term

Purpose: Anti-Collagen II/COL2A1 Antibody Picoband®

Sequence: AFAGLGQREK GPDPMQYMRA

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Collagen II/COL2A1 Antibody (ABIN5518709). Tested in IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of mouse Collagen II1222-1241aa AFAGLGQREKGPDPMQYMRA), different from the related rat sequence by one amino acid.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Rat, Mouse
Immunohistochemistry (Frozen Section), 0.5-1 μg/mL, Rat, Mouse
1. Donahue, L. R., Chang, B., Mohan, S., Miyakoshi, N., Wergedal, J. E., Baylink, D. J., Hawes, N. L., Rosen, C. J., Ward-Bailey, P., Zheng, Q. Y., Bronson, R. T., Johnson, K. R., Davisson, M. T. A missense mutation in the mouse Col2a1 gene causes spondyloepiphyseal dysplasia congenita, hearing loss, and retinoschisis. J. Bone Miner. Res. 18: 1612-1621, 2003. Note: Erratum: J. Bone Miner. Res. 22: 2011 only, 2007. 2. Faivre, L., Le Merrer, M., Douvier, S., Laurent, N., Thauvin-Robinet, C., Rousseau, T., Vereecke, I., Sagot, P., Delezoide, A.-L., Coucke, P., Mortier, G. Recurrence of achondrogenesis type II within the same family: evidence for germline mosaicism. Am. J. Med. Genet. 126A: 308-312, 2004.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(F).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Sodium azide, Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

References for anti-COL2A1 antibody (ABIN5518709)

Jia, Ma, Lv, Ma, Xu, Yang, Tian, Wang, Sun, Xu, Fu, Zhao: "Oestrogen and parathyroid hormone alleviate lumbar intervertebral disc degeneration in ovariectomized rats and enhance Wnt/β-catenin pathway activity." in: Scientific reports, Vol. 6, pp. 27521, (2018) (PubMed).

Li, Zou, Wang, Wang, Li, Chen, Sun, Sun: "TGF-β1/Smad3 Signaling Pathway Mediates T-2 Toxin-Induced Decrease of Type II Collagen in Cultured Rat Chondrocytes." in: Toxins, Vol. 9, Issue 11, (2018) (PubMed).

Liang, Sun, Wei, Zhang, Li, Xu, Shang, Deng, Zhao, Wei: "Establishment of rat ankle post-traumatic osteoarthritis model induced by malleolus fracture." in: BMC musculoskeletal disorders, Vol. 18, Issue 1, pp. 464, (2018) (PubMed).

Qin, Jin, Jiang, Li, Tan, Wu, Zheng, Zhao: "A Human Chondrocyte-Derived In Vitro Model of Alcohol-Induced and Steroid-Induced Femoral Head Necrosis." in: Medical science monitor : international medical journal of experimental and clinical research, Vol. 24, pp. 539-547, (2018) (PubMed).

Jiang, Zhong, Zheng, Zhao: "Nano-hydroxyapatite/collagen film as a favorable substrate to maintain the phenotype and promote the growth of chondrocytes cultured in vitro." in: International journal of molecular medicine, Vol. 41, Issue 4, pp. 2150-2158, (2018) (PubMed).

Zeng, Xiao, Deng, Li: "Chondroprotective Effects and Multitarget Mechanisms of Fu Yuan Capsule in a Rat Osteoarthritis Model." in: Evidence-based complementary and alternative medicine : eCAM, Vol. 2017, pp. 8985623, (2017) (PubMed).

Huang, Wu, Wang, Zheng, Zhao: "Protective effects of baicalin on rabbit articular chondrocytes in vitro." in: Experimental and therapeutic medicine, Vol. 13, Issue 4, pp. 1267-1274, (2017) (PubMed).

Hou, Huang, Luo, Wang, Liu, Deng, Zhang, Liu, Chen: "MiR-351 negatively regulates osteoblast differentiation of MSCs induced by (+)-cholesten-3-one through targeting VDR." in: American journal of translational research, Vol. 9, Issue 11, pp. 4963-4973, (2017) (PubMed).

Tian, Zhong, Zheng, Zhang, Liu, Zhang, Liu: "Neuroleukin/Autocrine Motility Factor Receptor Pathway Promotes Proliferation of Articular Chondrocytes through Activation of AKT and Smad2/3." in: Scientific reports, Vol. 5, pp. 15101, (2016) (PubMed).

Liu, Tian, Zhou, Wang, Gou, Zhang, Wang, Shen, Zhang, Zhang: "Protective effect of calcitonin on lumbar fusion-induced adjacent-segment disc degeneration in ovariectomized rat." in: BMC musculoskeletal disorders, Vol. 16, pp. 342, (2016) (PubMed).

Tian, Zhong, Zhang, Yin, Zhang, Liu: "Microfluidics‑based optimization of neuroleukin‑mediated regulation of articular chondrocyte proliferation." in: Molecular medicine reports, Vol. 13, Issue 1, pp. 67-74, (2016) (PubMed).

Gu, Li, Li, Yu, Wang, Ding, Liu: "Oral Resveratrol Prevents Osteoarthritis Progression in C57BL/6J Mice Fed a High-Fat Diet." in: Nutrients, Vol. 8, Issue 4, pp. 233, (2016) (PubMed).

Li, Sun, Liu, Chen, Rong, Lou, Yang, Yang, Liu: "Use of a biological reactor and platelet-rich plasma for the construction of tissue-engineered bone to repair articular cartilage defects." in: Experimental and therapeutic medicine, Vol. 12, Issue 2, pp. 711-719, (2016) (PubMed).

Liu, Lu, Wu, Zheng: "Chondroprotective effects of taurine in primary cultures of human articular chondrocytes." in: The Tohoku journal of experimental medicine, Vol. 235, Issue 3, pp. 201-13, (2015) (PubMed).

Ye, Wu, Li, Li, Zheng, Liu, Ye: "Millimeter Wave Treatment Inhibits Apoptosis of Chondrocytes via Regulation Dynamic Equilibrium of Intracellular Free Ca (2+)." in: Evidence-based complementary and alternative medicine : eCAM, Vol. 2015, pp. 464161, (2015) (PubMed).

Cai, Zhang, Zhang, Ge, Dai, Tan, Roodrajeetsing, Cai: "Promoting Effects on Proliferation and Chondrogenic Differentiation of Bone Marrow-Derived Mesenchymal Stem Cells by Four "Kidney-Tonifying" Traditional Chinese Herbs." in: BioMed research international, Vol. 2015, pp. 792161, (2015) (PubMed).

Sun, Meng, Li, Liu, Chen: "Construction of tissue-engineered laryngeal cartilage with a hollow, semi-flared shape using poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) as a scaffold." in: Experimental and therapeutic medicine, Vol. 9, Issue 4, pp. 1482-1488, (2015) (PubMed).

Wang, Yao, Huang, Li, Xu: "Effects of TGF-β1 and alginate on the differentiation of rabbit bone marrow-derived mesenchymal stem cells into a chondrocyte cell lineage." in: Experimental and therapeutic medicine, Vol. 10, Issue 3, pp. 995-1002, (2015) (PubMed).

Ouyang, Wang, Hong, Xin, Wang, Yang, Wang, Wang, Wei, Wang, Cui: "Establishment of a rat model for osteoarthritis resulting from anterior cruciate ligament rupture and its significance." in: Experimental and therapeutic medicine, Vol. 10, Issue 6, pp. 2035-2038, (2015) (PubMed).

Liu, Liu, Lin, Wei, Zheng: "Effect of JEZTC, a synthetic compound, on proliferation and phenotype maintenance of rabbit articular chondrocytes in vitro." in: In vitro cellular & developmental biology. Animal, Vol. 50, Issue 10, pp. 982-91, (2014) (PubMed).

Target Details for COL2A1

Target: COL2A1 (Collagen, Type II, alpha 1 (COL2A1))

Alternative Name: COL2A1

Background:

Synonyms: Collagen alpha-1 (II) chain,Alpha-1 type II collagen,Collagen alpha-1 (II) chain,Chondrocalcin,Col2a1,

Background: Collagen, type II, alpha 1, also known as COL2A1, is a human gene which is the major collagen synthesized by chondrocvtes. COL2A1 is mapped to 12q13.11. This gene encodes the alpha-1 chain of type II collagen, a fibrillar collagen found in cartilage and the vitreous humor of the eye. Mutations in this gene are associated with achondrogenesis, chondrodysplasia, early onset familial osteoarthritis, SED congenita, Langer-Saldino achondrogenesis, Kniest dysplasia, Stickler syndrome type I, and spondyloepimetaphyseal dysplasia Strudwick type. In addition, defects in processing chondrocalcin, a calcium binding protein that is the C-propeptide of this collagen molecule, are also associated with chondrodysplasia.

Sequence Similarities: Belongs to the fibrillar collagen family.

Molecular Weight: 170 kDa

UniProt: P28481

Pathways: Sensory Perception of Sound, Growth Factor Binding