ADAM28 antibody (N-Term)

Catalog No. ABIN5518723

The Rabbit Polyclonal anti-ADAM28 antibody has been validated for WB. It is suitable to detect ADAM28 in samples from Human.

Quick Overview for ADAM28 antibody (N-Term) (ABIN5518723)

Target: ADAM28 (ADAM Metallopeptidase Domain 28 (ADAM28))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ADAM28 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-ADAM28 Antibody

Binding Specificity: AA 207-248, N-Term

Purpose: Anti-ADAM28 Antibody Picoband®

Sequence: EYYLVLDNGE FKRYNENQDE IRKRVFEMAN YVNMLYKKLN TH

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-ADAM28 Antibody Picoband® (ABIN5518723). Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human ADAM28, different from the related mouse sequence by eleven amino acids.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human
1. "Entrez Gene: ADAM28 ADAM metallopeptidase domain 28". 2. Bridges LC, Hanson KR, Tani PH, et al. (2003). "Integrin alpha4beta1-dependent adhesion to ADAM 28 (MDC-L) requires an extended surface of the disintegrin domain". Biochemistry. 42 (13): 3734-41. 3. Howard L, Maciewicz RA, Blobel CP (2000). "Cloning and characterization of ADAM28: evidence for autocatalytic pro-domain removal and for cell surface localization of mature ADAM28". Biochem. J. 348 Pt 1 (Pt 1): 21-7.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for ADAM28

Target: ADAM28 (ADAM Metallopeptidase Domain 28 (ADAM28))

Alternative Name: ADAM28

Background:

Synonyms: Disintegrin and metalloproteinase domain-containing protein 28,ADAM 28,3.4.24.-,Epididymal metalloproteinase-like, disintegrin-like, and cysteine-rich protein II,eMDC II,Metalloproteinase-like, disintegrin-like, and cysteine-rich protein L,MDC-L,ADAM28,ADAM23, MDCL,

Tissue Specificity: Expressed predominantly in secondary lymphoid tissues, such as lymph node, spleen, small intestine, stomach, colon, appendix and trachea. The lymphocyte population is responsible for expression of this protein in these tissues. Isoform 2 is expressed preferentially in spleen.

Background: Disintegrin and metalloproteinase domain-containing protein 28 is an enzyme that in humans is encoded by the ADAM28 gene. This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. The protein encoded by this gene is a lymphocyte-expressed ADAM protein. And this gene is present in a gene cluster with other members of the ADAM family on chromosome 8. Alternative splicing results in multiple transcript variants.

Molecular Weight: 87 kDa, 61 kDa

Gene ID: 10863