Angiopoietin 1 antibody (Middle Region)

Catalog No. ABIN5518731

This Rabbit Polyclonal antibody specifically detects Angiopoietin 1 in WB and ELISA. It exhibits reactivity toward Humanand has been mentioned in 1 publication.

Quick Overview for Angiopoietin 1 antibody (Middle Region) (ABIN5518731)

Target: Angiopoietin 1 (ANGPT1)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Angiopoietin 1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-Angiopoietin 1 Antibody

Binding Specificity: AA 204-241, Middle Region

Purpose: Anti-Angiopoietin-1/ANGPT1 Antibody Picoband®

Sequence: KHKEELDTLK EEKENLQGLV TRQTYIIQEL EKQLNRAT

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Angiopoietin-1/ANGPT1 Antibody Picoband® (ABIN5518731). Tested in ELISA, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human Angiopoietin-1, different from the related mouse sequence by three amino acids, and from the related rat sequence by two amino acids.

Isotype: IgG

Application Details

Application Notes: ELISA, 0.1-0.5 μg/mL, -
Western blot, 0.1-0.5 μg/mL, Human
1. "Entrez Gene: ANGPT1 angiopoietin 1". 2. Cheung, A. H., Stewart, R. J., Marsden, P. A. Endothelial Tie2/Tek ligands angiopoietin-1 (ANGPT1) and angiopoietin-2 (ANGPT2): regional localization of the human genes to 8q22.3-q23 and 8p23. Genomics 48: 389-391, 1998. 3. Suri, C., Jones, P. F., Patan, S., Bartunkova, S., Maisonpierre, P. C., Davis, S., Sato, T. N., Yancopoulos, G. D. Requisite role of angiopoietin-1, a ligand for the TIE2 receptor, during embryonic angiogenesis. Cell 87: 1171-1180, 1996.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

References for anti-Angiopoietin 1 antibody (ABIN5518731)

Sun, Wang, Cheng, Wang, Li, Xue, Wang, Ai, Zhang, Xu, Wang, Ji: "Morroniside improves microvascular functional integrity of the neurovascular unit after cerebral ischemia." in: PLoS ONE, Vol. 9, Issue 6, pp. e101194, (2014) (PubMed).

Target Details for Angiopoietin 1

Target: Angiopoietin 1 (ANGPT1)

Alternative Name: ANGPT1

Background:

Synonyms: Angiopoietin-1,ANG-1,ANGPT1,KIAA0003,

Tissue Specificity: Widely expressed.

Background: Angiopoietin 1 is a type of angiopoietin and is encoded by the gene ANGPT1. Angiopoietins are proteins with important roles in vascular development and angiogenesis. All angiopoietins bind with similar affinity to an endothelial cell-specific tyrosine-protein kinase receptor. Angiopoietin 1 is mapped to 8q23.1. The protein encoded by this gene is a secreted glycoprotein that activates the receptor by inducing its tyrosine phosphorylation. It plays a critical role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme. The protein also contributes to blood vessel maturation and stability, and may be involved in early development of the heart. Angiopoietin-1 seems to play a crucial role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme.

Molecular Weight: 70 kDa

Gene ID: 284

UniProt: Q15389

Pathways: RTK Signaling, Glycosaminoglycan Metabolic Process