CKAP4 antibody (N-Term)

Catalog No. ABIN5518750

The Rabbit Polyclonal anti-CKAP4 antibody has been validated for WB. It is suitable to detect CKAP4 in samples from Mouse.

Quick Overview for CKAP4 antibody (N-Term) (ABIN5518750)

Target: CKAP4 (Cytoskeleton-Associated Protein 4 (CKAP4))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CKAP4 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-CKAP4 Antibody

Binding Specificity: AA 95-118, N-Term

Purpose: Anti-CKAP4 Antibody Picoband®

Sequence: LVAAAAFSGW YVHHVLEEVQ QVRR

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-CKAP4 Antibody Picoband® (ABIN5518750). Tested in WB applications. This antibody reacts with Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of mouse CKAP4, different from the related human sequence by one amino acid.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Mouse
1. "Entrez Gene: CKAP4 cytoskeleton-associated protein 4". 2. Schweizer A, Rohrer J, Jeno P, DeMaio A, Buchman TG, Hauri HP (Jul 1993). "A reversibly palmitoylated resident protein (p63) of an ER-Golgi intermediate compartment is related to a circulatory shock resuscitation protein". J Cell Sci. 104 (3): 685-94.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for CKAP4

Target: CKAP4 (Cytoskeleton-Associated Protein 4 (CKAP4))

Alternative Name: Ckap4

Background:

Synonyms: Cytoskeleton-associated protein 4,63- kDa cytoskeleton-linking membrane protein,Climp-63,p63,Ckap4,

Tissue Specificity: High in intestine and in peripheral blood mononuclear cells, low in lung, kidney, thymus, uterus, vasculature and brain. Not found in liver, heart, retina oe skeletal muscle.

Background: Cytoskeleton-associated protein 4, also known as CLIMP63 and p63, is a protein that in humans is encoded by the CKAP4 gene. It is mapped to 12q23.3. CKAP4 is found on the plasma membrane of select cells such as vascular smooth muscle and Type II Greater alveolar lung cells. It has a bimodal distribution. First, CKAP4 is found on the plasma membrane and serves as a receptor for SP-A/surfactant protein-A (in lung) and tPA (in vessels). Second, it is embedded in the membrane of a compartment that links the ER with the Golgi apparatus. This localization is dependent on its ability to form homooligomers, and its presence serves to anchor microtubules and the formation of tubular ER.

Molecular Weight: 66 kDa

Gene ID: 216197