GAPDH antibody (AA 136-335)

Catalog No. ABIN5518757

This anti-GAPDH antibody is a Rabbit Polyclonal antibody detecting GAPDH in WB, IHC, IF, ICC and FACS. Suitable for Human, Mouse, Rat, Chicken, Monkey and Zebrafish (Danio rerio). This Primary Antibody has been cited in 115+ publications.

Quick Overview for GAPDH antibody (AA 136-335) (ABIN5518757)

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Reactivity: Human, Mouse, Rat, Chicken, Monkey, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GAPDH antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-GAPDH Antibody

Binding Specificity: AA 136-335

Purpose: Anti-GAPDH Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-GAPDH Antibody Picoband® (ABIN5518757). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat, Monkey, Chicken, Zebrafish. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human GAPDH recombinant protein (Position: N136-E335). Human GAPDH shares 95% and 94.5% amino acid (aa) sequence identity with mouse and rat GAPDH, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Monkey, Mouse, Rat, Chicken, Zebrafish
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry(Fixed), 1-3 μg/1x10⁶ cells, Human
1. Bae, B.-I., Hara, M. R., Cascio, M. B., Wellington, C. L., Hayden, M. R., Ross, C. A., Ha, H. C., Li, X.-J., Snyder, S. H., Sawa, A. Mutant Huntingtin: nuclear translocation and cytotoxicity mediated by GAPDH. Proc. Nat. Acad. Sci. 103: 3405-3409, 2006. 2. Colell, A., Ricci, J.-E., Tait, S., Milasta, S., Maurer, U., Bouchier-Hayes, L., Fitzgerald, P., Guio-Carrion, A., Waterhouse, N. J., Li, C. W., Mari, B., Barbry, P., Newmeyer, D. D., Beere, H. M., Green, D. R. GAPDH and autophagy preserve survival after apoptotic cytochrome c release in the absence of caspase activation. Cell 129: 983-997, 2007. Note: Erratum: Cell 130: 385 only, 2007. 3. Tarze A, Deniaud A, Le Bras M, Maillier E, Molle D, Larochette N, Zamzami N, Jan G, Kroemer G, Brenner C (April 2007). "GAPDH, a novel regulator of the pro-apoptotic mitochondrial membrane permeabilization".Oncogene 26 (18): 2606-20.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

References for anti-GAPDH antibody (ABIN5518757)

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Liu, Kuang, Wu, Jin, Sun: "A novel polysaccharide from Sargassum integerrimum induces apoptosis in A549 cells and prevents angiogensis in vitro and in vivo." in: Scientific reports, Vol. 6, pp. 26722, (2018) (PubMed).

Zou, Duan, Wang, Gao, Chen, Ou, Liang, Wang, Yuan, Wang, Zhu: "DYT-40, a novel synthetic 2-styryl-5-nitroimidazole derivative, blocks malignant glioblastoma growth and invasion by inhibiting AEG-1 and NF-κB signaling pathways." in: Scientific reports, Vol. 6, pp. 27331, (2018) (PubMed).

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Hu, Cao, Pang, Luo, Zou, Feng, Li, Chen, Huang: "Tumor grade related expression of neuroglobin is negatively regulated by PPARγ and confers antioxidant activity in glioma progression." in: Redox biology, Vol. 12, pp. 682-689, (2018) (PubMed).

Liao, Xing, Xu, Liu, Zhang: "Evaluation of the circulating level of fibroblast activation protein α for diagnosis of esophageal squamous cell carcinoma." in: Oncotarget, Vol. 8, Issue 18, pp. 30050-30062, (2018) (PubMed).

Cai, Tan, Li, Gao, Li, Li, Tang, Li, Hui: "Glucagon-Like Peptide-1 (GLP-1) Treatment Ameliorates Cognitive Impairment by Attenuating Arc Expression in Type 2 Diabetic Rats." in: Medical science monitor : international medical journal of experimental and clinical research, Vol. 23, pp. 4334-4342, (2018) (PubMed).

Wu, Sun, Ye, Zhang, Guo, Huang, Jing, Xiang, Yu, Guo: "Iguratimod prevents ovariectomy‑induced bone loss and suppresses osteoclastogenesis via inhibition of peroxisome proliferator‑activated receptor‑γ." in: Molecular medicine reports, Vol. 16, Issue 6, pp. 8200-8208, (2018) (PubMed).

Liu, Liu, Tan, Tang, Long, Li, Liang, Chu, Yang: "Gaseous signalling molecule SO2 via Hippo‑MST pathway to improve myocardial fibrosis of diabetic rats." in: Molecular medicine reports, Vol. 16, Issue 6, pp. 8953-8963, (2018) (PubMed).

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Miao, Margolis, Gao, Voss, Li, Martini, Norton, Bossé, Wankowicz, Cullen, Horak, Wind-Rotolo, Tracy, Giannakis, Hodi, Drake, Ball, Allaf, Snyder, Hellmann, Ho, Motzer, Signoretti, Kaelin, Choueiri et al.: "Genomic correlates of response to immune checkpoint therapies in clear cell renal cell carcinoma. ..." in: Science (New York, N.Y.), Vol. 359, Issue 6377, pp. 801-806, (2018) (PubMed).

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Target Details for GAPDH

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Alternative Name: GAPDH

Background:

Synonyms: Glyceraldehyde-3-phosphate dehydrogenase,GAPDH,1.2.1.12,Peptidyl-cysteine S-nitrosylase GAPDH,2.6.99.-,GAPDH,GAPD,CDABP0047, OK/SW-cl.12,

Tissue Specificity: Highly expressed in metastatic colon tumors. Expressed in primary colon tumors. Weakly expressed in normal colon tissue.

Background: Glyceraldehyde 3-phosphate dehydrogenase (abbreviated as GAPDH or less commonly as G3PDH) is an enzyme of ~37 kDa that catalyzes the sixth step of glycolysis and thus serves to break down glucose for energy and carbon molecules. This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. GAPDH is mapped to 12p13.31. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus.

Molecular Weight: 36 kDa

Gene ID: 2597

UniProt: P04406