NOS2 antibody (C-Term)

Catalog No. ABIN5518782

This Rabbit Polyclonal antibody specifically detects NOS2 in WB. It exhibits reactivity toward Human and has been mentioned in 17+ publications.

Quick Overview for NOS2 antibody (C-Term) (ABIN5518782)

Target: NOS2 (Nitric Oxide Synthase 2, Inducible (NOS2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NOS2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-NOS2 Antibody

Binding Specificity: AA 1088-1126, C-Term

Purpose: Anti-iNOS/NOS2 Antibody Picoband®

Sequence: ARDVAHTLKQ LVAAKLKLNE EQVEDYFFQL KSQKRYHED

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-iNOS/NOS2 Antibody Picoband® (ABIN5518782). Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human iNOS, different from the related mouse sequence by five amino acids, and from the related rat sequence by four amino acids.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human
1. Chartrain, N. A., Geller, D. A., Koty, P. P., Sitrin, N. F., Nussler, A. K., Hoffman, E. P., Billiar, T. R., Hutchinson, N. I., Mudgett, J. S. Molecular cloning, structure, and chromosomal localization of the human inducible nitric oxide synthase gene. J. Biol. Chem. 269: 6765-6772, 1994. 2. Diefenbach, A., Schindler, H., Rollinghoff, M., Yokoyama, W. M., Bogdan, C. Requirement for type 2 NO synthase for IL-12 signaling in innate immunity. Science 284: 951-955, 1999. 3. Hobbs, M. R., Udhayakumar, V., Levesque, M. C., Booth, J., Roberts, J. M., Tkachuk, A. N., Pole, A., Coon, H., Kariuki, S., Nahlen, B. L., Mwaikambo, E. D., Lai, A. L., Granger, D. L., Anstey, N. M., Weinberg, J. B. A new NOS2 promoter polymorphism associated with increased nitric oxide production and protection from severe malaria in Tanzanian and Kenyan children. Lancet 360: 1468-1475, 2002.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

References for anti-NOS2 antibody (ABIN5518782)

Liu, Zhang, Han, Wang, Liu, Zhang, Zhou, Xiang: "[Corrigendum] Inhibition of BTK protects lungs from trauma-hemorrhagic shock-induced injury in rats." in: Molecular medicine reports, Vol. 17, Issue 5, pp. 6926, (2018) (PubMed).

Sun, Yang, Zhang, Zhao: "Esculentoside A ameliorates cecal ligation and puncture-induced acute kidney injury in rats." in: Experimental animals, (2017) (PubMed).

Wang, Chai, He, Ai, Sun, Huang, Li: "Intermittent hypoxia simulating obstructive sleep apnea causes pulmonary inflammation and activates the Nrf2/HO-1 pathway." in: Experimental and therapeutic medicine, Vol. 14, Issue 4, pp. 3463-3470, (2017) (PubMed).

Huang, Chen, Wang, Wang, Ning, He, Hu, Yuan, Li, Wang, Liu, Chen, Ren, Sun: "Detecting cell-in-cell structures in human tumor samples by E-cadherin/CD68/CD45 triple staining." in: Oncotarget, Vol. 6, Issue 24, pp. 20278-87, (2016) (PubMed).

Lin, Sun, Wang, Zheng, Zhang, Zheng: "Celastrol Ameliorates Ulcerative Colitis-Related Colorectal Cancer in Mice via Suppressing Inflammatory Responses and Epithelial-Mesenchymal Transition." in: Frontiers in pharmacology, Vol. 6, pp. 320, (2016) (PubMed).

Dhillon, Mastropaolo, Murchie, Griffiths, Thöni, Elkadri, Xu, Mack, Walters, Guo, Mack, Huynh, Baksh, Silverberg, Brumell, Snapper, Muise: "Higher activity of the inducible nitric oxide synthase contributes to very early onset inflammatory bowel disease." in: Clinical and translational gastroenterology, Vol. 5, pp. e46, (2014) (PubMed).

Zhao, Xiong, Mao, Meng, Lei, Li, Deng, Chen, Tu, Lu, Yang, He: "Atmospheric pressure room temperature plasma jets facilitate oxidative and nitrative stress and lead to endoplasmic reticulum stress dependent apoptosis in HepG2 cells." in: PLoS ONE, Vol. 8, Issue 8, pp. e73665, (2013) (PubMed).

Long, Yu, Shuai, Guo, Duan, Xu, Li: "The hypoglycemic effect of the kelp on diabetes mellitus model induced by alloxan in rats." in: International journal of molecular sciences, Vol. 13, Issue 3, pp. 3354-65, (2012) (PubMed).

Zhang, Wei, Wang, Ding, Wang, Shi: "Cell-specific expression and immunolocalization of nitric oxide synthase isoforms and the related nitric oxide/cyclic GMP signaling pathway in the ovaries of neonatal and immature rats." in: Journal of Zhejiang University. Science. B, Vol. 12, Issue 1, pp. 55-64, (2011) (PubMed).

Wang, Du, Wang, Kuang, Wang: "Z-ligustilide attenuates lipopolysaccharide-induced proinflammatory response via inhibiting NF-kappaB pathway in primary rat microglia." in: Acta pharmacologica Sinica, Vol. 31, Issue 7, pp. 791-7, (2010) (PubMed).

Wu, Yin, Yao, Haefliger: "Inhibition by brimonidine of forskolin-induced nitric oxide synthase expression in human ciliary bodies in vitro." in: Molecular vision, Vol. 13, pp. 493-6, (2007) (PubMed).

Sun, Han, Jia, Jiang, Wang, Zhang, Han, Jiang: "Expressions of inducible nitric oxide synthase and matrix metalloproteinase-9 and their effects on angiogenesis and progression of hepatocellular carcinoma." in: World journal of gastroenterology, Vol. 11, Issue 38, pp. 5931-7, (2006) (PubMed).

Wang, Ji, Wang, Gu: "Anti-cancer effect of iNOS inhibitor and its correlation with angiogenesis in gastric cancer." in: World journal of gastroenterology, Vol. 11, Issue 25, pp. 3830-3, (2005) (PubMed).

Xu, Deng, Zhu, Lin: "Role of inducible nitric oxide synthase expression in aberrant crypt foci-adenoma-carcinoma sequence." in: World journal of gastroenterology, Vol. 9, Issue 6, pp. 1246-50, (2003) (PubMed).

Sassenroth, Trinkmann: "[Implantation of a Binkhorst lens in chronic simple glaucoma]." in: Fortschritte der Ophthalmologie : Zeitschrift der Deutschen Ophthalmologischen Gesellschaft, Vol. 82, Issue 2, pp. 186, (1985) (PubMed).

Shapiro: "Portrait of a pioneer laryngologist." in: Eye, ear, nose & throat monthly, Vol. 53, Issue 11, pp. 460-4, (1975) (PubMed).

Achten: "Diseases of the dermis. General considerations." in: Archives belges de dermatologie et de syphiligraphie, Vol. 28, Issue 1, pp. 1-13, (1973) (PubMed).

Target Details for NOS2

Target: NOS2 (Nitric Oxide Synthase 2, Inducible (NOS2))

Alternative Name: NOS2

Background:

Synonyms: Nitric oxide synthase, inducible,1.14.13.39,Hepatocyte NOS,HEP-NOS,Inducible NO synthase,Inducible NOS,iNOS,N

Tissue Specificity: Expressed in the liver, retina, bone cells and airway epithelial cells of the lung. Not expressed in the platelets.

Background: Nitric oxide synthase, inducible is an enzyme that in humans is encoded by the NOS2 gene. Nitric oxide (NO) is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter, it is implicated in neurotoxicity associated with stroke and neurodegenerative diseases, neural regulation of smooth muscle, including peristalsis, and penile erection. Three different NOS isoforms have been identified which fall into two distinct types, constitutive and inducible. The inducible NOS (iNOS) isoform is expressed in a variety of cell types and tissues in response to inflammatory agents and cytokines. The human iNOS (NOS2) gene is approximately 37 kb in length and consists of 26 exons and 25 introns. NOS2-derived NO is a prerequisite for cytokine signaling and function in innate immunity.

Molecular Weight: 131 kDa

Gene ID: 4843

UniProt: P35228

Pathways: Retinoic Acid Receptor Signaling Pathway, Cellular Response to Molecule of Bacterial Origin, Inositol Metabolic Process, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process