CA4 antibody (AA 19-284)

Catalog No. ABIN5518815

This Rabbit Polyclonal antibody specifically detects CA4 in WB. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for CA4 antibody (AA 19-284) (ABIN5518815)

Target: CA4 (Carbonic Anhydrase IV (CA4))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CA4 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-CA4 Antibody

Binding Specificity: AA 19-284

Purpose: Anti-Carbonic Anhydrase IV/CA4 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Carbonic Anhydrase IV/CA4 Antibody Picoband® (ABIN5518815). Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human CA IV recombinant protein (Position: A19-S284). Human CA IV shares 55.5% and 58.9% amino acid (aa) sequence identity with mouse and rat CA IV, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Mouse, Rat, Human
1. Okuyama T, Batanian JR, Sly WS (Aug 1993). "Genomic organization and localization of gene for human carbonic anhydrase IV to chromosome 17q". Genomics. 16 (3): 678-84. 2. Sterling D, Alvarez BV, Casey JR (Jul 2002). "The extracellular component of a transport metabolon. Extracellular loop 4 of the human AE1 Cl-/HCO3- exchanger binds carbonic anhydrase IV". J. Biol. Chem. 277 (28): 25239-46.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for CA4

Target: CA4 (Carbonic Anhydrase IV (CA4))

Alternative Name: CA4

Background:

Synonyms: Carbonic anhydrase 4,4.2.1.1,Carbonate dehydratase IV,Carbonic anhydrase IV,CA-IV,CA4,

Tissue Specificity: Expressed in the endothelium of the choriocapillaris in eyes (at protein level). Not expressed in the retinal epithelium at detectable levels. .

Background: Carbonic anhydrase 4 is an enzyme that in humans is encoded by the CA4 gene. Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. This gene encodes a glycosylphosphatidyl-inositol-anchored membrane isozyme expressed on the luminal surfaces of pulmonary (and certain other) capillaries and proximal renal tubules. Its exact function is not known, however, it may have a role in inherited renal abnormalities of bicarbonate transport.

Molecular Weight: 35 kDa

Gene ID: 762

UniProt: P22748