HFE antibody (AA 82-199)

Catalog No. ABIN5518842

This Rabbit Polyclonal antibody specifically detects HFE in WB. It exhibits reactivity toward Human.

Quick Overview for HFE antibody (AA 82-199) (ABIN5518842)

Target: HFE (Hemochromatosis (HFE))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HFE antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-HFE Antibody

Binding Specificity: AA 82-199

Purpose: Anti-HFE Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-HFE Antibody Picoband® (ABIN5518842). Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human HFE recombinant protein (Position: Q82-R199). Human HFE shares 72.2% and 74.6% amino acid (aa) sequence identity with mouse and rat HFE, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human
1. Martins, R, Silva, B, Proen99a, D, Faustino, P (3 March 2011). "Differential HFE gene expression is regulated by alternative splicing in human tissues". PLOS ONE. 6 (3): e17542. 2. Waheed, A, Parkkila, S, Saarnio, J, Fleming, RE, Zhou, XY, Tomatsu, S, Britton, RS, Bacon, BR, Sly, WS (16 February 1999). "Association of HFE protein with transferrin receptor in crypt enterocytes of human duodenum". Proceedings of the National Academy of Sciences of the United States of America. 96 (4): 1579-84.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for HFE

Target: HFE (Hemochromatosis (HFE))

Alternative Name: HFE

Background:

Synonyms: Hereditary hemochromatosis protein,HLA-H,HFE,HLAH,

Tissue Specificity: Expressed in all tissues tested except brain.

Background: Human hemochromatosis protein also known as the HFE protein is a protein which in humans is encoded by the HFE gene. The HFE gene is located on short arm of chromosome 6 at location 6p21.3. The protein encoded by this gene is a membrane protein that is similar to MHC class I-type proteins and associates with beta2-microglobulin (beta2M). It is thought that this protein functions to regulate iron absorption by regulating the interaction of the transferrin receptor with transferrin. The iron storage disorder, hereditary haemochromatosis, is a recessive genetic disorder that results from defects in this gene.

Molecular Weight: 40 kDa

Gene ID: 3077

UniProt: Q30201

Pathways: Transition Metal Ion Homeostasis, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process