Sca-1/Ly-6A/E antibody (Middle Region)

Catalog No. ABIN5518854

This Rabbit Polyclonal antibody specifically detects Sca-1/Ly-6A/E in FACS, WB and IHC. It exhibits reactivity toward Mouseand has been mentioned in 1 publication.

Quick Overview for Sca-1/Ly-6A/E antibody (Middle Region) (ABIN5518854)

Target: Sca-1/Ly-6A/E (Ly6a) (Lymphocyte Antigen 6 Complex, Locus A (Ly6a))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Sca-1/Ly-6A/E antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-Sca-1/Ly-6A/E Antibody

Binding Specificity: AA 48-75, Middle Region

Purpose: Anti-Sca1/Ly6A/E Antibody Picoband®

Sequence: YPDGVCVTQE AAVIVDSQTR KVKNNLCL

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Sca1/Ly6A/E Antibody Picoband® (ABIN5518854). Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of mouse Sca1/Ly6A/E.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Mouse
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Mouse
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Mouse
1. Codias, E.K. et al. (1990) J. Immunol. 144:2197. 2. Malek, T.R. et al. (1986) J. Exp. Med. 164:709 .

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

References for anti-Sca-1/Ly-6A/E antibody (ABIN5518854)

Xiang, Liang, Zhen-Yong, Liang-Jun, Zhong-Liang: "BMP9-induced osteogenetic differentiation and bone formation of muscle-derived stem cells." in: Journal of biomedicine & biotechnology, Vol. 2012, pp. 610952, (2012) (PubMed).

Target Details for Sca-1/Ly-6A/E

Target: Sca-1/Ly-6A/E (Ly6a) (Lymphocyte Antigen 6 Complex, Locus A (Ly6a))

Alternative Name: Ly6a

Background:

Synonyms: Lymphocyte antigen 6A-2/6E-1,Ly-6A.2/Ly-6E.1,Stem cell antigen 1,SCA-1,T-cell-activating protein,TAP,Ly6a,Ly6,

Tissue Specificity: Widely expressed. .

Background: Stem cell antigen-1 (Sca-1) is a mouse glycosyl phosphatidylinositol-anchored protein and a cell surface marker found on hematopoietic stem cells (HSCs). It is encoded by the strain-specific Ly-6 E/A allelic gene. Sca-1 has been discovered in several non hematopoietic tissues and can be used to enrich progenitor cell populations other than HSC. It is suggested that Sca-1 could be involved in regulating both B and T cell activation.

Molecular Weight: 14 kDa

Gene ID: 110454

UniProt: P05533

Pathways: Sensory Perception of Sound, Activated T Cell Proliferation