NCKAP1 antibody (AA 2-201)

Catalog No. ABIN5518856

This anti-NCKAP1 antibody is a Rabbit Polyclonal antibody detecting NCKAP1 in WB. Suitable for Human, Mouse and Rat.

Quick Overview for NCKAP1 antibody (AA 2-201) (ABIN5518856)

Target: NCKAP1 (NCK Associated Protein 1 (NCKAP1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NCKAP1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-NCKAP1 Antibody

Binding Specificity: AA 2-201

Purpose: Anti-NCKAP1 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-NCKAP1 Antibody Picoband® (ABIN5518856). Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human NCKAP1 recombinant protein (Position: S2-K201). Human NCKAP1 shares 98.5% and 98% amino acid (aa) sequence identity with mouse and rat NCKAP1, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
1. Eden S, Rohatgi R, Podtelejnikov AV, Mann M, Kirschner MW (Aug 2002). "Mechanism of regulation of WAVE1-induced actin nucleation by Rac1 and Nck". Nature. 418 (6899): 790-3. 2. Kitamura Y, Kitamura T, Sakaue H, Maeda T, Ueno H, Nishio S, Ohno S, Osada SI, Sakaue M, Ogawa W, Kasuga M (Mar 1997). "Interaction of Nck-associated protein 1 with activated GTP-binding protein Rac". The Biochemical Journal. 322 (Pt 3): 873-8. 3. Suzuki, T., Nishiyama, K., Yamamoto, A., Inazawa, J., Iwaki, T., Yamada, T., Kanazawa, I., Sakaki, Y. Molecular cloning of a novel apoptosis-related gene, human Nap1 (NCKAP1), and its possible relation to Alzheimer disease. Genomics 63: 246-254, 2000.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for NCKAP1

Target: NCKAP1 (NCK Associated Protein 1 (NCKAP1))

Alternative Name: NCKAP1

Background:

Synonyms: Nck-associated protein 1,NAP 1,Membrane-associated protein HEM-2,p125Nap1,NCKAP1,HEM2, KIAA0587, NAP1,

Tissue Specificity: Expressed in all tissues examined except peripheral blood leukocytes, with highest expression in brain, heart, and skeletal muscle.

Background: Nck-associated protein 1 is a protein that in humans is encoded by the NCKAP1 gene. By somatic cell hybrid analysis, the NCKAP1 gene was mapped to chromosome 2. NCKAP1 is a predicted type II transmembrane protein, with a C-terminal transmembrane domain. It was demonstrated that suppression of NCKAP1 expression using an antisense NCKAP1 oligonucleotide induced apoptosis of neuronal cells.

Molecular Weight: 129 kDa

Gene ID: 10787

UniProt: Q9Y2A7

Pathways: RTK Signaling, Tube Formation, Asymmetric Protein Localization, Embryonic Body Morphogenesis