CD90 antibody (AA 20-131)

Catalog No. ABIN5518877

This anti-CD90 antibody is a Rabbit Polyclonal antibody detecting CD90 in WB, IHC and IF. Suitable for Mouse and Rat. This Primary Antibody has been cited in 2+ publications.

Quick Overview for CD90 antibody (AA 20-131) (ABIN5518877)

Target: CD90 (THY1)

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD90 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-CD90 Antibody

Binding Specificity: AA 20-131

Purpose: Anti-CD90/Thy1 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-CD90/Thy1 Antibody Picoband® (ABIN5518877). Tested in IF, IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived mouse CD90/Thy1 recombinant protein (Position: Q20-C131). Mouse CD90/Thy1 shares 63.4% and 81.3% amino acid (aa) sequence identity with human and rat CD90/Thy1, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Mouse, Rat
Immunofluorescence, 5 μg/mL, Rat
1. Abeysinghe, H. R., Cao, Q., Xu, J., Pollock, S., Veyberman, Y., Guckert, N. L., Keng, P., Wang, N.THY1 expression is associated with tumor suppression of human ovarian cancer.Cancer Genet. Cytogenet. 143: 125-132, 2003. 2. McKenzie, J. L., Fabre, J. W.Human Thy-1: unusual localization and possible functional significance in lymphoid tissues.J. Immun. 126: 843-850, 1981. 3. Morris, R.Thy-1 in developing nervous tissue.Dev. Neurosci. 7: 133-160, 1985.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

References for anti-CD90 antibody (ABIN5518877)

Tian, Tao, Zhao, Tai, Liu, Liu: "Isolation and morphological characterization of ovine amniotic fluid mesenchymal stem cells." in: Experimental animals, Vol. 65, Issue 2, pp. 125-34, (2017) (PubMed).

Zhang, Shang, Hao, Zheng, Li, Liang, Cui, Liu: "Effects of human umbilical cord mesenchymal stem cell transplantation combined with minimally invasive hematoma aspiration on intracerebral hemorrhage in rats." in: American journal of translational research, Vol. 7, Issue 11, pp. 2176-86, (2016) (PubMed).

Target Details for CD90

Target: CD90 (THY1)

Alternative Name: Thy1

Background:

Synonyms: Thy-1 membrane glycoprotein,Thy-1 antigen,CD90,Thy1,Thy-1,

Tissue Specificity: Detected adjacent to nodes of Ranvier in juxtaparanodal zones in spinal cord nerve fibers, but also in paranodal regions in some myelinated spinal cord axons (at protein level) (PubMed:11086297). Detected in the islet of Langerhans (PubMed:21483673).

Background: CD90 (Cluster of Differentiation 90) or Thy-1 is a 25-37 kDa heavily N-glycosylated, glycophosphatidylinositol (GPI) anchored conserved cell surface protein with a single V-like immunoglobulin domain, originally discovered as a thymocyte antigen. The CD90 gene is mapped to 11q23.3. Thy-1 can be used as a marker for a variety of stem cells and for the axonal processes of mature neurons. Structural study of Thy-1 lead to the foundation of the Immunoglobulin superfamily, of which it is the smallest member, and led to the first biochemical description and characterization of a vertebrate GPI anchor.

Molecular Weight: 22 kDa

Gene ID: 21838

UniProt: P01831

Pathways: Cell-Cell Junction Organization