TIMP3 antibody (Middle Region)

Catalog No. ABIN5518878

This Rabbit Polyclonal antibody specifically detects TIMP3 in WB and ELISA. It exhibits reactivity toward Human, Mouse and Rat and has been mentioned in 2+ publications.

Quick Overview for TIMP3 antibody (Middle Region) (ABIN5518878)

Target: TIMP3 (TIMP Metallopeptidase Inhibitor 3 (TIMP3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TIMP3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-TIMP3 Antibody

Binding Specificity: AA 107-141, Middle Region

Purpose: Anti-TIMP3 Antibody Picoband®

Sequence: RVYDGKMYTG LCNFVERWDQ LTLSQRKGLN YRYHL

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-TIMP3 Antibody Picoband® (ABIN5518878). Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human TIMP3, different from the related mouse and rat sequences by two amino acids.

Isotype: IgG

Application Details

Application Notes: ELISA, 0.1-0.5 μg/mL, -
Western blot, 0.1-0.5 μg/mL, Mouse, Rat, Human
1. "Entrez Gene: TIMP3 TIMP metallopeptidase inhibitor 3 (Sorsby fundus dystrophy, pseudoinflammatory)". 2. Apte, S. S., Mattei, M.-G., Olsen, B. R. : Cloning of the cDNA encoding human tissue inhibitor of metalloproteinases-3 (TIMP-3) and mapping of the TIMP3 gene to chromosome 22. Genomics 19: 86-90, 1994. 3. Qi JH, Ebrahem Q, Moore N, Murphy G, Claesson-Welsh L, Bond M, Baker A, Anand-Apte B (Apr 2003). "A novel function for tissue inhibitor of metalloproteinases-3 (TIMP3): inhibition of angiogenesis by blockage of VEGF binding to VEGF receptor-2". Nat Med 9 (4): 407-15.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

References for anti-TIMP3 antibody (ABIN5518878)

Guan, Zhang, Song, Dai: "Promoter methylation and expression of TIMP3 gene in gastric cancer." in: Diagnostic pathology, Vol. 8, pp. 110, (2014) (PubMed).

Bai, Yi, Li, Sui: "Clinicopathologic significance of BAG1 and TIMP3 expression in colon carcinoma." in: World journal of gastroenterology, Vol. 13, Issue 28, pp. 3883-5, (2007) (PubMed).

Target Details for TIMP3

Target: TIMP3 (TIMP Metallopeptidase Inhibitor 3 (TIMP3))

Alternative Name: TIMP3

Background:

Synonyms: Metalloproteinase inhibitor 3,Protein MIG-5,Tissue inhibitor of metalloproteinases 3,TIMP-3,TIMP3,

Tissue Specificity: Ubiquitously expressed.

Background: Metalloproteinase inhibitor 3 is a protein that in humans is encoded by the TIMP3 gene. It is mapped to 22q12.1-q13.2. This gene belongs to the tissue inhibitor of metalloproteinases gene family. The proteins encoded by this gene family are inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of theextracellular matrix (ECM). Expression of this gene is induced in response to mitogenic stimulation and this netrin domain-containing protein is localized to the ECM. Mutations in this gene have been associated with the autosomal dominant disorder Sorsby's fundus dystrophy.

Molecular Weight: 24 kDa

UniProt: P35625