ATOH1 antibody (N-Term)

Catalog No. ABIN5518893

This Rabbit Polyclonal antibody specifically detects ATOH1 in WB. It exhibits reactivity toward Human and Rat.

Quick Overview for ATOH1 antibody (N-Term) (ABIN5518893)

Target: ATOH1 (Atonal Homolog 1 (Drosophila) (ATOH1))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATOH1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-ATOH1 Antibody

Binding Specificity: AA 1-30, N-Term

Purpose: Anti-MATH1/HATH1/ATOH1 Antibody Picoband®

Sequence: MSRLLHAEEW AEVKELGDHH RQPQPHHLPQ

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-MATH1/HATH1/ATOH1 Antibody Picoband® (ABIN5518893). Tested in WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human MATH1/HATH1, different from the related mouse sequence by three amino acids.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Rat
1. Ben-Arie N, McCall AE, Berkman S, Eichele G, Bellen HJ, Zoghbi HY (Sep 1996). "Evolutionary conservation of sequence and expression of the bHLH protein Atonal suggests a conserved role in neurogenesis". Human Molecular Genetics. 5 (9): 1207-16. 2. "Entrez Gene: ATOH1 atonal homolog 1 (Drosophila)".

Comment:

We recommend Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ABIN921124) for Western blot.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for ATOH1

Target: ATOH1 (Atonal Homolog 1 (Drosophila) (ATOH1))

Alternative Name: ATOH1

Background:

Synonyms: Protein atonal homolog 1,Class A basic helix-loop-helix protein 14,bHLHa14,Helix-loop-helix protein hATH-1,hATH1,ATOH1,ATH1, BHLHA14,

Tissue Specificity: Retina.

Background: Protein atonal homolog 1 is a protein that in humans is encoded by the ATOH1 gene. This protein belongs to the basic helix-loop-helix (BHLH) family of transcription factors. It activates E-box dependent transcription along with E47. ATOH1 is required for the formation of both neural and non-neural cell types. Using genetic deletion in mice, Atoh1 has been shown to be essential for formation of cerebellar granule neurons, inner ear hair cells, spinal cord interneurons, Merkel cells of the skin, and intestinal secretory cells (goblet, enteroendocrine, and Paneth cells).

Molecular Weight: 38 kDa

Gene ID: 474

UniProt: Q92858