FABP4 antibody (AA 2-132)

Catalog No. ABIN5518913

This anti-FABP4 antibody is a Rabbit Polyclonal antibody detecting FABP4 in WB and ELISA. Suitable for Mouse and Rat.

Quick Overview for FABP4 antibody (AA 2-132) (ABIN5518913)

Target: FABP4 (Fatty Acid Binding Protein 4, Adipocyte (FABP4))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FABP4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-FABP4 Antibody

Binding Specificity: AA 2-132

Purpose: Anti-FABP4 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-FABP4 Antibody Picoband® (ABIN5518913). Tested in ELISA, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived rat FABP4 recombinant protein (Position: C2-A132). Rat FABP4 shares 88.5% and 93.9% amino acid (aa) sequence identity with human and mouse FABP4, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Mouse, Rat
ELISA, 0.1-0.5 μg/mL,
1. Hotamisligil, G. S., Johnson, R. S., Distel, R. J., Ellis, R., Papaioannou, V. E., Spiegelman, B. M. : Uncoupling of obesity from insulin resistance through a targeted mutation in aP2, the adipocyte fatty acid binding protein. Science 274: 1377-1379, 1996. 2. Furuhashi, M., Tuncman, G., Gorgun, C. Z., Makowski, L., Atsumi, G., Vaillancourt, E., Kono, K., Babaev, V. R., Fazio, S., Linton, M. F., Sulsky, R., Robl, J. A., Parker, R. A., Hotamisligil, G. S. : Treatment of diabetes and atherosclerosis by inhibiting fatty-acid-binding protein aP2. Nature 447: 959-965, 2007. 3. Shum, B. O. V., Mackay, C. R., Gorgun, C. Z., Frost, M. J., Kumar, R. K., Hotamisligil, G. S., Rolph, M. S. : The adipocyte fatty acid-binding protein aP2 is required in allergic airway inflammation. J. Clin. Invest. 116: 2183-2192, 2006.

Comment:

We recommend Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ABIN921124) for Western blot.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for FABP4

Target: FABP4 (Fatty Acid Binding Protein 4, Adipocyte (FABP4))

Alternative Name: Fabp4

Background:

Synonyms: Fatty acid-binding protein, adipocyte,Adipocyte lipid-binding protein,ALBP,Adipocyte-type fatty acid-binding protein,A-FABP,AFABP,Fatty acid-binding protein 4,Fabp4,

Tissue Specificity: Expressed in the endothelium of the choriocapillaris in eyes (at protein level). Not expressed in the retinal epithelium at detectable levels. .

Background: Fatty acid binding proteins (FABPs) are small cytoplasmic proteins that are expressed in a highly tissue-specific manner and bind to fatty acids such as oleic and retinoic acid. Adipocyte fatty-acid-binding protein, aP2 (FABP4) is expressed in adipocytes and macrophages, and integrates inflammatory and metabolic responses. Studies in aP2-deficient mice have shown that this lipid chaperone has a significant role in several aspects of metabolic syndrome, including type 2 diabetes and atherosclerosis. It regulates allergic airway inflammation and may provide a link between fatty acid metabolism and asthma.

Molecular Weight: 15 kDa

Gene ID: 79451

UniProt: P70623

Pathways: Brown Fat Cell Differentiation