HSPB6 antibody (AA 73-123)

Catalog No. ABIN5518920

This Rabbit Polyclonal antibody specifically detects HSPB6 in WB, IHC, IF and ICC. It exhibits reactivity toward Human, Rat and Mouse.

Quick Overview for HSPB6 antibody (AA 73-123) (ABIN5518920)

Target: HSPB6 (Heat Shock Protein, alpha-Crystallin-Related, B6 (HSPB6))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HSPB6 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-HSPB6 Antibody

Binding Specificity: AA 73-123

Purpose: Anti-Hsp20/HSPB6 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Hsp20/HSPB6 Antibody Picoband® (ABIN5518920). Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived human Hsp20 recombinant protein (Position: H73-L123). Human Hsp20 shares 88.2% and 92.2% amino acid (aa) sequence identity with mouse and rat Hsp20, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
1. "Entrez Gene: HSPB6 heat shock protein, alpha-crystallin-related, B6". 2. Bukach OV, Seit-Nebi AS, Marston SB, Gusev NB (Jan 2004). "Some properties of human small heat shock protein Hsp20 (HspB6)". Eur J Biochem. 271 (2): 291-302. 3. Dreiza CM, Komalavilas P, Furnish EJ, Flynn CR, Sheller MR, Smoke CC, Lopes LB, Brophy CM (Jan 2010)."The small heat shock protein, HSPB6, in muscle function and disease". Cell Stress Chaperones. 15 (1): 1-11.

Comment:

We recommend Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ABIN921124) for Western blot.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for HSPB6

Target: HSPB6 (Heat Shock Protein, alpha-Crystallin-Related, B6 (HSPB6))

Alternative Name: HSPB6

Background:

Synonyms: Heat shock protein beta-6,HspB6,Heat shock 20 kDa-like protein p20,HSPB6,

Tissue Specificity: Mainly expressed in testis. Expressed to a lower level in thymus. Weakly or not expressed in heart, liver, brain, placenta, lung, skeletal muscle, kidney and pancreas.

Background: Heat shock protein beta-6 is a protein that in humans is encoded by the HSPB6 gene. HSPB6, also known as hsp20, is a 17- kDa member of the small heat shock family of proteins. It is expressed in multiple tissues, however, HSPB6 is most highly and constitutively expressed in different types of muscle including vascular, airway, colonic, bladder, uterine smooth muscle, cardiac muscle and skeletal muscle. HSPB6 has specific functions for vasodilation, platelet function, and insulin resistance and in smooth and cardiac muscle.

Molecular Weight: 17 kDa

Gene ID: 126393

UniProt: O14558