IL-6 Receptor antibody (C-Term)

Catalog No. ABIN5518926

This Rabbit Polyclonal antibody specifically detects IL-6 Receptor in WB. It exhibits reactivity toward Human.

Quick Overview for IL-6 Receptor antibody (C-Term) (ABIN5518926)

Target: IL-6 Receptor (IL6R) (Interleukin 6 Receptor (IL6R))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL-6 Receptor antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-IL-6 Receptor Antibody

Binding Specificity: AA 379-419, C-Term

Purpose: Anti-IL6R Antibody Picoband®

Sequence: LLCIAIVLRF KKTWKLRALK EGKTSMHPPY SLGQLVPERP R

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-IL6R Antibody Picoband® (ABIN5518926). Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human IL6R.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human
1. Hoek KS, Schlegel NC, Eichhoff OM, Widmer DS, Praetorius C, Einarsson SO, Valgeirsdottir S, Bergsteinsdottir K, Schepsky A, Dummer R, Steingrimsson E (2008). "Novel MITF targets identified using a two-step DNA microarray strategy". Pigment Cell Melanoma Res. 21 (6): 665-76. 2. Schwantner A, Dingley AJ, Ozbek S, Rose-John S, Grötzinger J (Jan 2004). " determination of the interleukin-6 binding epitope of the interleukin-6 receptor by NMR spectroscopy". J. Biol. Chem. 279 (1): 571-6.

Comment:

We recommend Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ABIN921124) for Western blot.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for IL-6 Receptor

Target: IL-6 Receptor (IL6R) (Interleukin 6 Receptor (IL6R))

Alternative Name: IL6R

Background:

Synonyms: Interleukin-6 receptor subunit alpha,IL-6 receptor subunit alpha,IL-6R subunit alpha,IL-6R-alpha,IL-6RA,IL-6R 1,Membrane glycoprotein 80,gp80,CD126,IL6R,

Tissue Specificity: Isoform 2 is expressed in peripheral blood mononuclear cells and weakly found in urine and serum.

Background: Interleukin 6 receptor (IL6R), also known as CD126 (Cluster of Differentiation 126), is a type I cytokine receptor. This gene encodes a subunit of the interleukin 6 (IL6) receptor complex. Interleukin 6 is a potent pleiotropic cytokine that regulates cell growth and differentiation and plays an important role in the immune response. The IL6 receptor is a protein complex consisting of this protein and interleukin 6 signal transducer (IL6ST/GP130/IL6-beta), a receptor subunit also shared by many other cytokines. Dysregulated production of IL6 and this receptor are implicated in the pathogenesis of many diseases, such as multiple myeloma, autoimmune diseases and prostate cancer. Alternatively spliced transcript variants encoding distinct isoforms have been reported. A pseudogene of this gene is found on chromosome 9.

Molecular Weight: 52 kDa

Gene ID: 3570

UniProt: P08887

Pathways: JAK-STAT Signaling, Autophagy, Growth Factor Binding, Cancer Immune Checkpoints