MAK antibody (C-Term)

Catalog No. ABIN5518933

The Rabbit Polyclonal anti-MAK antibody has been validated for WB. It is suitable to detect MAK in samples from Human.

Quick Overview for MAK antibody (C-Term) (ABIN5518933)

Target: MAK (Male Germ Cell-Associated Kinase (MAK))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MAK antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-MAK Antibody

Binding Specificity: AA 588-623, C-Term

Purpose: Anti-MAK Antibody Picoband®

Sequence: RTYNPTAKNL NIVNRAQPIP SVHGRTDWVA KYGGHR

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-MAK Antibody Picoband® (ABIN5518933). Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human MAK, different from the related mouse and rat sequences by two amino acids.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human
1. Ma AH, Xia L, Desai SJ, Boucher DL, Guan Y, Shih HM, Shi XB, deVere White RW, Chen HW, Tepper CG, Kung HJ (Sep 2006). "Male germ cell-associated kinase, a male-specific kinase regulated by androgen, is a coactivator of androgen receptor in prostate cancer cells". Cancer Res. 66 (17): 8439-47. 2. Matsushime H, Jinno A, Takagi N, Shibuya M (1990)."A novel mammalian protein kinase gene (mak) is highly expressed in testicular germ cells at and after meiosis". Mol. Cell. Biol. 10 (5): 2261-8.

Comment:

We recommend Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ABIN921124) for Western blot.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for MAK

Target: MAK (Male Germ Cell-Associated Kinase (MAK))

Alternative Name: MAK

Background:

Synonyms: Serine/threonine-protein kinase MAK,2.7.11.22,Male germ cell-associated kinase,MAK,

Tissue Specificity: Expressed in prostate cancer cell lines at generally higher levels than in normal prostate epithelial cell lines. Isoform 1 is expressed in kidney, testis, lung, trachea, and retina. Isoform 2 is retina-specific where it is expressed in rod and cone photoreceptors. .

Background: Serine/threonine-protein kinase MAK is an enzyme that in humans is encoded by the MAK gene. The product of this gene is a serine/threonine protein kinase related to kinases involved in cell cycle regulation. Studies of the mouse and rat homologs have localized the kinase to the chromosomes during meiosis in spermatogenesis, specifically to the synaptonemal complex that exists while homologous chromosomes are paired. Mutations in this gene have been associated with ciliary defects resulting in retinitis pigmentosa 62.

Molecular Weight: 70 kDa

Gene ID: 4117

UniProt: P20794