Myoglobin antibody (N-Term)

Catalog No. ABIN5518936

Product Details anti-Myoglobin Antibody

Target: Myoglobin (MB)

Binding Specificity: AA 3-35, N-Term

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Myoglobin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Purpose: Anti-Myoglobin/MB Antibody Picoband®

Sequence: LSDGEWQLVL NVWGKVEADI PGHGQEVLIR LFK

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Myoglobin(MB) detection. Tested with WB in Human.
Gene Name: myoglobin
Protein Name: Myoglobin

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human Myoglobin, different from the related mouse sequence by three amino acids, and from the related rat sequence by six amino acids.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry, 2-5 μg/mL, Human, Mouse, Rat
1. Boyer, S. H., Fainer, D. C., Naughton, M. A. Myoglobin inherited structural variation in man. Science 140: 1228-1231, 1963. 2. Boulton, F. E., Huntsman, R. G., Lorkin, P. A., Lehmann, H. Abnormal human myoglobin: 53(D4) glutamic acid lysine. Nature 223: 832-833, 1969. 3. Jeffreys, A. J., Wilson, V., Blanchetot, A., Weller, P., Geurts van Kessel, A., Spurr, N., Solomon, E., Goodfellow, P. The human myoglobin gene: a third dispersed globin locus in the human genome. Nucleic Acids Res. 12: 3235-3243, 1984.

Comment:

We recommend Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ABIN921124) for Western blot.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for Myoglobin

Target: Myoglobin (MB)

Alternative Name: Myoglobin (MB Products)

Background:

Synonyms: Myoglobin,MB,

Tissue Specificity: Brain and spinal cord. Isoform 1 is also detected in testis, liver, colon and ovary.

Background: Myoglobin (MB) also known as PVALB, is a single-chain globular protein of 153 or 154 amino acids, containing a heme (iron-containing porphyrin) prosthetic group in the center around which the remaining apoprotein folds. It is a member of the globin superfamily and is expressed in skeletal and cardiac muscles. This gene is mapped to chromosome 22q11-q13. Myoglobin is released from damaged muscle tissue (rhabdomyolysis), which has very high concentrations of myoglobin. The released myoglobin is filtered by the kidneys but is toxic to the renal tubular epithelium and so may cause acute renal failure.

Molecular Weight: 17 kDa

Gene ID: 4151

UniProt: P02144

Pathways: Brown Fat Cell Differentiation