SRY antibody (Middle Region)

Catalog No. ABIN5518959

This Rabbit Polyclonal antibody specifically detects SRY in WB. It exhibits reactivity toward Human.

Quick Overview for SRY antibody (Middle Region) (ABIN5518959)

Target: SRY (Sex Determining Region Y (SRY))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SRY antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-SRY Antibody

Binding Specificity: AA 90-130, Middle Region

Purpose: Anti-SRY Antibody Picoband®

Sequence: ISKQLGYQWK MLTEAEKWPF FQEAQKLQAM HREKYPNYKY R

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-SRY Antibody Picoband® (ABIN5518959). Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human SRY.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human
1. Biason-Lauber A, Konrad D, Meyer M, DeBeaufort C, Schoenle EJ (May 2009). "Ovaries and female phenotype in a girl with 46,XY karyotype and mutations in the CBX2 gene". Am. J. Hum. Genet. 84 (5): 658-63. 2. Margarit E, Coll MD, Oliva R, Gómez D, Soler A, Ballesta F (January 2000). "SRY gene transferred to the long arm of the X chromosome in a Y-positive XX true hermaphrodite". Am. J. Med. Genet. 90 (1): 25-8. 3. Polanco JC, Koopman P (February 2007). "Sry and the hesitant beginnings of male development". Dev. Biol. 302 (1): 13-24.

Comment:

We recommend Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ABIN921124) for Western blot.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for SRY

Target: SRY (Sex Determining Region Y (SRY))

Alternative Name: SRY

Background:

Synonyms: Sex-determining region Y protein,Testis-determining factor,SRY,TDF,

Tissue Specificity: Plasma. Isoform 1 and isoform 2 are widely expressed. Isoform 1 is the predominant form in spleen. .

Background: This intronless gene encodes a transcription factor that is a member of the high mobility group (HMG)-box family of DNA-binding proteins. This protein is the testis-determining factor (TDF), which initiates male sex determination. Mutations in this gene give rise to XY females with gonadal dysgenesis (Swyer syndrome), translocation of part of the Y chromosome containing this gene to the X chromosome causes XX male syndrome.

Molecular Weight: 26 kDa

Gene ID: 6736

UniProt: Q05066