VSX2 antibody (AA 249-361)

Catalog No. ABIN5518966

The Rabbit Polyclonal anti-VSX2 antibody has been validated for WB. It is suitable to detect VSX2 in samples from Human and Mouse.

Quick Overview for VSX2 antibody (AA 249-361) (ABIN5518966)

Target: VSX2 (Visual System Homeobox 2 (VSX2))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This VSX2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-VSX2 Antibody

Binding Specificity: AA 249-361

Purpose: Anti-CHX10/VSX2 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-CHX10/VSX2 Antibody Picoband® (ABIN5518966). Tested in WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived human CHX10 recombinant protein (Position: A249-A361). Human CHX10 shares 90.3% amino acid (aa) sequence identity with mouse CHX10.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Mouse, Human
1. McInnes, R. R., Basu, S., Novak, J., Ploder, L., Liang, M.-Y., Hawes, N., Taylor, B., Roderick, T., Goldman, D., Hankin, M., Burmeister, M. The ocular retardation (orJ) mouse has an ochre mutation in the homeobox gene Chx10: evidence for Chx10 as a major determinant of retinal development. (Abstract) Am. J. Hum. Genet. 55 (suppl.): A3, 1994. 2. Reichman, S., Kalathur, R. K. R., Lambard, S., Ait-Ali, N., Yang, Y., Lardenois, A., Ripp, R., Poch, O., Zack, D. J., Sahel, J.-A., Leveillard, T. The homeobox gene CHX10/VSX2 regulates RdCVF promoter activity in the inner retina. Hum. Molec. Genet. 19: 250-261, 2010.

Comment:

We recommend Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ABIN921124) for Western blot.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for VSX2

Target: VSX2 (Visual System Homeobox 2 (VSX2))

Alternative Name: VSX2

Background:

Synonyms: Visual system homeobox 2,Ceh-10 homeodomain-containing homolog,Homeobox protein CHX10,VSX2,CHX10, HOX10,

Tissue Specificity: Abundantly expressed in retinal neuroblasts during eye development and in the inner nuclear layer of the adult retina. Within this layer, expression is stronger in the outer margin where bipolar cells predominate.

Background: Visual system homeobox 2 is a protein that in humans is encoded by the VSX2 gene. This gene encodes a homeobox protein originally described as a retina-specific transcription factor. Mutations in this gene are associated with microphthalmia, cataracts and iris abnormalities. In situ hybridization to human fetal retinal sections detected CHX10 expression in retinal neuroblasts at all stages examined. Human CHX10 is expressed in progenitor cells of the developing neuroretina and in the inner nuclear layer of the mature retina.

Molecular Weight: 43 kDa

Gene ID: 338917

UniProt: P58304

Pathways: Dopaminergic Neurogenesis