XDH antibody (AA 2-343)

Catalog No. ABIN5518967

This anti-XDH antibody is a Rabbit Polyclonal antibody detecting XDH in WB, IHC, ICC, FACS and IHC (fro). Suitable for Human, Mouse and Rat. This Primary Antibody has been cited in 1 publication.

Quick Overview for XDH antibody (AA 2-343) (ABIN5518967)

Target: XDH (Xanthine Dehydrogenase (XDH))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This XDH antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-XDH Antibody

Binding Specificity: AA 2-343

Purpose: Anti-Xanthine Oxidase/XDH Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Xanthine Oxidase/XDH Antibody Picoband® (ABIN5518967). Tested in Flow Cytometry, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived human Xanthine Oxidase recombinant protein (Position: T2-K343). Human Xanthine Oxidase shares 86.8% and 89.2% amino acid (aa) sequence identity with mouse and rat Xanthine Oxidase, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Mouse, Rat, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Mouse, Rat, Human
Immunohistochemistry (Frozen Section), 0.5-1 μg/mL, Human
Immunocytochemistry, 0.5-1 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human1. Ichida K, Amaya Y, Noda K, Minoshima S, Hosoya T, Sakai O, Shimizu N, Nishino T (Nov 1993). "Cloning of the cDNA encoding human xanthine dehydrogenase (oxidase): structural analysis of the protein and chromosomal location of the gene". Gene. 133 (2): 279-84. 2. Parzen SD, Fox AS (Dec 1964). "Purification of xanthine dehydrogenase from drosophila melanogaster". BiochimicaetBiophysicaActa. 92: 465-71.

Comment:

We recommend Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ABIN921124) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

References for anti-XDH antibody (ABIN5518967)

Huang, Zhu, Li, Sui, Min: "Effect of Blueberry Anthocyanins Malvidin and Glycosides on the Antioxidant Properties in Endothelial Cells." in: Oxidative medicine and cellular longevity, Vol. 2016, pp. 1591803, (2017) (PubMed).

Target Details for XDH

Target: XDH (Xanthine Dehydrogenase (XDH))

Alternative Name: XDH

Background:

Synonyms: Xanthine dehydrogenase/oxidase,Xanthine dehydrogenase,XD,1.17.1.4 ,Xanthine oxidase,XO,1.17.3.2 ,Xanthine oxidoreductase,XOR,XDH,XDHA,

Tissue Specificity: Detected in milk (at protein level). .

Background: Xanthine dehydrogenase, also known as XDH, is a protein that, in humans, is encoded by the XDH gene. Xanthine dehydrogenase belongs to the group of molybdenum-containing hydroxylases involved in the oxidative metabolism of purines. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Xanthine dehydrogenase can be converted to xanthine oxidase by reversible sulfhydryl oxidation or by irreversible proteolytic modification. Defects in xanthine dehydrogenase cause xanthinuria, may contribute to adult respiratory stress syndrome, and may potentiate influenza infection through an oxygen metabolite-dependent mechanism.

Molecular Weight: 146 kDa

Gene ID: 7498

UniProt: P47989

Pathways: Positive Regulation of Endopeptidase Activity