OB Cadherin antibody (AA 55-293)

Catalog No. ABIN5518978

The Rabbit Polyclonal anti-OB Cadherin antibody has been validated for WB, ELISA, IHC and FACS. It is suitable to detect OB Cadherin in samples from Human, Mouse and Rat.

Quick Overview for OB Cadherin antibody (AA 55-293) (ABIN5518978)

Target: OB Cadherin (CDH11) (Cadherin 11 (CDH11))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This OB Cadherin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

Product Details anti-OB Cadherin Antibody

Binding Specificity: AA 55-293

Purpose: Anti-OB Cadherin/CDH11 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-OB Cadherin/CDH11 Antibody Picoband® (ABIN5518978). Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human OB Cadherin/CDH11 recombinant protein (Position: K371-S796).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Kremmidiotis G, Baker E, Crawford J, Eyre HJ, Nahmias J, Callen DF (Aug 1998). "Localization of human cadherin genes to chromosome regions exhibiting cancer-related loss of heterozygosity". Genomics. 49 (3): 467-71. 2. Vogt L, Schrimpf SP, Meskenaite V, Frischknecht R, Kinter J, Leone DP, Ziegler U, Sonderegger P (2001). "Calsyntenin-1, a proteolytically processed postsynaptic membrane protein with a cytoplasmic calcium-binding domain". Mol. Cell. Neurosci. 17 (1): 151-66.

Comment:

We recommend Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ABIN921124) for Western blot.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for OB Cadherin

Target: OB Cadherin (CDH11) (Cadherin 11 (CDH11))

Alternative Name: CDH11

Background:

Synonyms: Tumor necrosis factor ligand superfamily member 18, Activation-inducible TNF-related ligand, AITRL, Glucocorticoid-induced TNF-related ligand, hGITRL, TNFSF18, AITRL, GITRL, TL6, UNQ149/PRO175,

Tissue Specificity: Expressed at high levels in the small intestine, ovary, testis, kidney and endothelial cells.

Background: Cadherin-11 is a protein that in humans is encoded by the CDH11 gene. This gene encodes a type II classical cadherin from the cadherin superfamily, integral membrane proteins that mediate calcium-dependent cell-cell adhesion. Mature cadherin proteins are composed of a large N-terminal extracellular domain, a single membrane-spanning domain, and a small, highly conserved C-terminal cytoplasmic domain. Type II (atypical) cadherins are defined based on their lack of a HAV cell adhesion recognition sequence specific to type I cadherins. Expression of this particular cadherin in osteoblastic cell lines, and its upregulation during differentiation, suggests a specific function in bone development and maintenance.

Molecular Weight: 120 kDa

Gene ID: 1009

UniProt: P55287