FGF7 antibody (AA 32-194)

Catalog No. ABIN5518984

Product Details anti-FGF7 Antibody

Target: FGF7 (Fibroblast Growth Factor 7 (FGF7))

Binding Specificity: AA 32-194

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FGF7 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Purpose: Anti-KGF/Fgf7 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-KGF/Fgf7 Antibody Picoband® (ABIN5518984). Tested in ELISA, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived mouse KGF recombinant protein (Position: C32-T194).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL
ELISA, 0.1-0.5 μg/mL
1. Kelley MJ, Pech M, Seuanez HN, Rubin JS, O'Brien SJ, Aaronson SA (Nov 1992). "Emergence of the keratinocyte growth factor multigene family during the great ape radiation". Proc Natl Acad Sci U S A 89 (19): 9287-91. 2. Mattei MG, deLapeyriere O, Bresnick J, Dickson C, Birnbaum D, Mason I (Jun 1995). "Mouse Fgf7 (fibroblast growth factor 7) and Fgf8 (fibroblast growth factor 8) genes map to chromosomes 2 and 19 respectively". Mamm Genome 6 (3): 196-7.

Comment:

We recommend Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ABIN921124) for Western blot.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

References for anti-FGF7 antibody (ABIN5518984)

Li, Liang, Zhao, Uchida, Baba, Huang, Bai, Bai, Zhang: "The effects of adenoviral transfection of the keratinocyte growth factor gene on epidermal stem cells: an in vitro study." in: Molecules and cells, Vol. 36, Issue 4, pp. 316-21, (2013) (PubMed).

Target Details for FGF7

Target: FGF7 (Fibroblast Growth Factor 7 (FGF7))

Alternative Name: Fgf7 (FGF7 Products)

Background:

Synonyms: Fibroblast growth factor 7, FGF-7, Heparin-binding growth factor 7, HBGF-7, Keratinocyte growth factor, KGF, Fgf7, Fgf-7, Kgf,

Tissue Specificity: Ubiquitous. Expressed in the rod and cone photoreceptors, extending from the tips of the outer segment (OS) through the inner segment (IS) and outer nuclear layer (ONL) and into the synaptic terminals of the outer plexiform layer (ONL). Also detected in the bipolar, horizontal and amacrine cells in the inner nuclear layer (INL), extending to the inner plexiform layer (IPL) and though the ganglion cell layer (GCL) and into the nerve fiber layer (NFL) (at protein level).

Background: Keratinocyte growth factor is a protein that in humans is encoded by the FGF7 gene. The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein is a potent epithelial cell-specific growth factor, whose mitogenic activity is predominantly exhibited in keratinocytes but not in fibroblasts and endothelial cells. Studies of mouse and rat homologs of this gene implicated roles in morphogenesis of epithelium, reepithelialization of wounds, hair development and early lung organogenesis.

Molecular Weight: 22 kDa

Gene ID: 14178

UniProt: P36363

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway