FLT3 antibody (AA 62-295)

Catalog No. ABIN5518988

The Rabbit Polyclonal anti-FLT3 antibody (ABIN5518988) specifically detects FLT3 in WB, ELISA and FACS. The antibody is reactive with Mouse and Rat samples.

Quick Overview for FLT3 antibody (AA 62-295) (ABIN5518988)

Target: FLT3 (Fms-Related tyrosine Kinase 3 (FLT3))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FLT3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-FLT3 Antibody

Binding Specificity: AA 62-295

Purpose: Anti-Flt3/CD135 Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Flt3/ Antibody (ABIN5518988). Tested in ELISA, IHC, WB applications. This antibody reacts with Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived mouse Flt3 / CD135 recombinant protein (Position: E62-E295).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Mouse ELISA, 0.1-0.5 μg/mL, -

Comment:

We recommend Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ABIN921124) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for FLT3

Target: FLT3 (Fms-Related tyrosine Kinase 3 (FLT3))

Alternative Name: Flt3

Background:

Synonyms: Receptor-type tyrosine-protein kinase FLT3, 2.7.10.1, FL cytokine receptor, Fetal liver kinase 2, FLK-2, Fms-like tyrosine kinase 3, FLT-3, Tyrosine-protein kinase receptor flk-2, CD135, Flt3, Flk-2, Flt-3,

Tissue Specificity: Hematopoietic stem and progenitor cell- enriched populations. Found in brain, placenta and testis.

Background: Cluster of differentiation antigen 135 (CD135) also known as fms like tyrosine kinase 3 (FLT-3), receptor-type tyrosine-protein kinase FLT3, or fetal liver kinase-2 (Flk2) is a protein that in humans is encoded by the FLT3 gene. This gene encodes a class III receptor tyrosine kinase that regulates hematopoiesis. This receptor is activated by binding of the fms-related tyrosine kinase 3 ligand to the extracellular domain, which induces homodimer formation in the plasma membrane leading to autophosphorylation of the receptor. The activated receptor kinase subsequently phosphorylates and activates multiple cytoplasmic effector molecules in pathways involved in apoptosis, proliferation, and differentiation of hematopoietic cells in bone marrow.

Molecular Weight: 150 kDa

Gene ID: 14255

UniProt: Q00342

Pathways: RTK Signaling